Lecture 14- techniques

Southern Blotting
- Cleave the DNA
- with restriction enzymesSeparate
- fragments on agarose gelTransfer
- fragments to support, like nitrocellulose, in same positionHybridize with a
- specific probe to detect certain DNA sequences/genes of interest
Northern Blotting
Similar to Southern Blotting, but bind and analyze RNA
Western Blotting

Separate the proteins by size (electrophoresis)

Bind antibodies to find proteins of interest
All techniques

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miingargiola

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Lecture 14- techniques

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Lecture 14- techniques

Updated

2010-08-25T00:10:05Z

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