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What are the two ways for cellular respiration?
Aerobic Respirations and Fermentation
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Process by which living cells obtain energy from organic molecules.
Cellular respiration
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What is the primary aim for respiration?
ATP and NADH
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What method of resp. uses O2 and put sout CO2?
Aerobic
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What is mainly used in aerobic resp.?
Glucose ( Glucose Metabolism)
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What are the four metabolic pathways?
Glycolysis, Breakdown of Pyrivate, and Oxidative Phosphorylation.
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What is the first stage of cellular resp.?
Glycolysis ( can occur w/ or w/o O2)
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Is Glycolysis the same for all organisms?
Yes
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What are the steps for Glycolysis?
Ten steps in three phases.
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What are the three phases in Glycolysis?
Energy investment, cleavage, and energy liberation
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What happens in the energy investment phase?
steps 1-3, 2ATO hydrolyzed to create fructose-1,6 bisphosphate
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What happens in the cleavage phase?
steps 4-5 6C broken into two 3 Glyceraldehyde-3-phosphate
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What happens in the energy liberation phase?
steps 6-10 2 glyceraldehyde-3-phosphate broken down into two pyuvate molecules. THis produces 2 NADH and 4ATP
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What is the net yield of Glycolysis?
2
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What is the second stage of cellular respiration?
Breakdown of pyruvate
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In eukararyotes where is the pyruvate transported?
Mitochondrial Matrix
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Molecule of CO2 removed from each pyruvate and Remaining is?
attached to CoA to make Acetyl CoA
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What is the yeild to stage 2 on cellular resp?
1NADH per pyruvate.
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Stage 3 of cellular resp.?
Metabolic cycles
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Stage 3: Acetyl is removed from acetyl CoA and attached to oxalocetate to form?
Citrate (Citric Acid)
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What is released in the stage 3?
2CO2, 1 ATP, 3NADH, and 1 FADH2
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In stage 3 what is regenerated to start the cycle again?
Oxaloacetate
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What happens in the 4th stage of cellular resp?
High energy e- removed from NADH and FADH2 to make ATP
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In stage 4 of cell resp: what causes the phosphorlation?
ATP synthase
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Oxidation by the Electron Transport Chain (ETC). Protein complexes and small organic molecules embedded in the inner mitochondrial membrane. Accept and donate electrons in a linear manner in a series of redox reactions. Movement of electrons generates an H+ electrochemical gradient (proton-motive force). This provides energy for the next step
synthesizing ATP
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Lipid bilayer of inner mitochondrial membrane is relatively impermeable to H+. Protons can only pass through
ATP synthase
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What is the yield in the 4th stage of cellular resp?
30-40 ATP molecules/glucose
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What other molecules can be used for the cellular resp in place of glucose?
Carbs, proteins, and fats
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fron enviroments that lack O2 what are the two strategies that can be used?
Use substance other than O2 as final electron acceptor in electron transport chain or Produce ATP only via substrate-level phosphorylation
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What does ecoli use for cell. resp?
Nitrate
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is the breakdown of organic molecules without net oxidation?
Fermentation
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In fermentation Many organisms can only use O2 as final electron acceptor, so under anaerobic conditions, they need a different way to produce ATP, like using glycolysis. But glycolysis uses up NAD+ and makes too much NADH under anaerobic conditions (dangerous situation).
Muscles reduce pyruvate into lactate and yest makes ethanol
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What happens in photosythesis?
CO2 is reduced and H2O oxidized and is an endergonic rxn
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regions on the surface of the Earth and atmosphere where living organisms exist. Largely driven by the photosynthetic power of green plants
Biosphere
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What are the tropic levels?
Heterotroph, auto troph, and photoautotroph
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Must eat food to sustain life
Heterotroph
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Makes organic molecules from inorganic sources.
Autotroph
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Use light as a source of energy. Green plants, algae, cyanobacteria
Photoautotroph
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Majority of photosynthesis occurs internally in leaves, in the
Mespphyll
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Carbon dioxide enters and oxygen exits leaf through pores called
stomata
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What is the atatomy of the chloroplast?
Outer and Inner membrane separated by an inner membrane. Thylakoid membrane, Granum and the fluid filled area called stroma
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Conatins the pigment molecules, forms thylakoids, and enclose the thylakoid lumen.
Thylakoid membrane
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A Stack of thylakoids
Granum
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Fluid filled region between thylakoid membrane and inner membrane
Stroma
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Two stages of photosynthesis
Light Rxns and Calvin cycle
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This stage of photosynthesis Use light energy, Take place in thylakoid membranes, Produce ATP, NADPH and O2
Light Rxns
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What do the light rxns yeild?
ATP, NADPH and O2
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This stage of photosynthesis Occurs in stroma and Uses ATP and NADPH to incorporate CO2 into carbohydrate
Calvin cylce
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Wavelengths that are absorbed by different pigments
Absorbtion spectrum
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Rate of photosynthesis by whole plant at specific wavelengths
Action spectrum
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Captured light energy can be transferred to other molecules to produce energy intermediate molecules for
Cellular work
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Thylakoid membranes of chloroplast contain two distinct complexes of molecules
Photosystem 1 and Photosystem 2
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Of the two photosystems which where discovered first?
Photosystem 1
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What are the steps in the Photosytem 2?
the initial step, excited E- go from PS2 to PS1. Water oxidizes into 02 and H+. released the energy in electron transport chain. That energy is used to make the H+ electrochem. gradient.
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What are the steps in photo system 1?
Primary role is to make NADPH. Addition of H+ to NADP+ contributes to the H+ gradient by de[leting H+ from the stroma.
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Achieved by chemiosmotic mechanism called photophosphorylation or Driven by flow of H+ from thylakoid lumen into stroma via
ATP synthesi in chloroplasts
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What are the 3 ways H+ gradient is generated?
in thylakoid lumen by splitting of water, by ETC pumping H+ into lumen, and in stroma from formation of NADPH.
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How is O2 produced in photsynthesis?
Produced in the thylakoid by oxidation of H2O by PS2. Two electrons transferred to P680* molecules.
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How is NADPH produced in Photosynthesis?
Stroma from high energy electrons that startin PS2 and are boosted in PS1. NADP+ + 2e- + H+ -> NADPH
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How is ATP produced in photosynthesis?
Produced in stroma by ATP synthase using the. H+ electrochemical gradient
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What are the two electron flows in the electron gradient?
Produced in stroma by ATP synthase using the H+ electrochemical gradient
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In this electron flow Electrons begin at PSII and eventually transfer to NADPH, a linear process. Produces both ATP and NADPH in equal amounts
Noncyclic
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In this electron flow Electrons begin at PSII and eventually transfer to NADPH, a linear process. Produces both ATP and NADPH in equal amounts
Cyclic photophosphorylation (cyclic electron flow)
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What are the two main components in the photosystem 2?
Light-harvesting complex (or antenna complex) and reaction center
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this component of photosystem 2 directlty absorbs photons and energy transferred via resonance energy transfer.
Light-harvesting complex
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THis component of photosystem 2 P680 -> P680* ( rather unstable for moves quick). Electrons transfered to primary electron acceptor and captured. Water is oxidized to replace the electron on p680* producing o2 gas in the process
Reaction center
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In this cycle CO2 incorporated into carbohydrates, requires a massive input og=f eneergy and produces glyceraldehyde-3-phosphate
Calvin cycle
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In the calvin cycle for every 6CO2 inc. what must be used?
18 ATO and 12 NADPH
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What is the product from the calvin cycle?
glyceraldehyde-3-phosphate (G3P) then later used to make glucose
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What are the three phases of the calvin cycle?
Carbon fixation, Reduction and carbohydrate production, and regeneration of RuBP.
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In this phase of the calvin cycle CO2 incorporated into RuBP using rubisco. Reaction product is a six-carbon intermediate that splits into two 3-phosphoglycerate molecules (3PG)
Phase 1 carbon fixation
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in this phase of the calvin cycle ATP is used to convert 3PG into 1,3-bisphosphoglycerate (1,3-BPG). NADPH electrons reduce it to glyceraldehyde-3-phosphate (G3P). 6 CO2 ? 12 G3P. Only 2 G3P molecules used for carbohydrates. 10 G3P molecules must be used for regeneration of RuBP
Phase 2 redction and carbohydrate production
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in this phase of the calvin cycle ? 10 G3P are converted into 6 RuBP using 6 ATP
Phase 3 regeneration of RuBP
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What conditions can effect the calvin cycle?
Light intensity, Temperature, Water availability
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Rubisco functions as a carboxylase. RuBP + CO2 ? 2 3PG. C3 plants make 3PG
Photorespiration
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These plants Evolved a mechanism to minimize respiration and make oxalocetate in the first step of carbon fixation.
c4 plants
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90% of plants are
c3 plants
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Some C4 plants separate processes using time. ? CO2 enters and is converted to malate. Stomata close during the day to conserve water. Oxaloacetate converted to malate. Malate broken down into CO2 to drive Calvin cycle during the day
Crassulacean Acid Metabolism aka CAM plants
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Cell communication is the process of cells detecting and responding to signals in the
Extracellular enviroment
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By changing the conformationof a receptor, signals lead to a responce inside?
The cell
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Signlas that cause cell death is?
Apoptosis
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Why do cells need signals?
To respond to a changing environment and to communicate with eachother
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This in plants allows multiple cells to change shape, bending the plant in coordinated way?
Phototropism.
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Signaling molecule in phototropism?
Auxin
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What are the signals relayed between cells?
Direct intercellular signaling, Contact-dependent signaling, Autocrine signaling, Paracrine signaling, and Endocrine signaling
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Cell junctions allow signaling molecules to pass from one cell to another
Direct intercellular signaling
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Molecules bound to the surface of cells serve as signals to cells coming in contact with them
Contact-dependent signaling
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Cells secrete signaling molecules that bind to their own cell surface or similar neighboring cells
Autocrine signaling
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Signal does not affect originating cell, but does influence nearby cells
Paracrine signaling
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Signals called hormones travel long distances and are usually longer lasting in effect
Endocrine signaling
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What are the three stages of cell signaling?
Receptor activation, signal transduction, and cellular responce.
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What are the different cellular responces change?
Enzyme activity, structural proteins, and gene expression
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Binds noncovalently to receptor with high specificity. It is the signaling molecule.
Ligand
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3 kinds of surface receptors.
Enzyme-linked receptors, G-protein coupled receptors (GPCR), and Ligand-gated ion channels
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Found in all living species. Extracellular domain binds signal . Intracellular domain becomes functional catalyst. Most are protein kinases
Enzyme-linked receptors
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Found in all eukaryotes, common in animals. 7 transmembrane segments. Activated receptor binds to G protein. Releases GDP and binds GTP instead. GTP causes G protein to dissociate. a subunit and ?/? dimer interact with other proteins in a signaling pathway
G-protein coupled receptors (GPCR)
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Plant and animal cells. Ligand binding causes ion channels to open and ions to flow through the membrane. In animals, these transmit synaptic signals between neurons and muscles or between two neurons
Ligand-gated ion channels
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These recpetors are inside the cell
Intracellular recptors
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the signaling molecule binds to cell surface receptor and the conformation change stimulates a signal transduction pathway.
produces the cellular responce
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Category of enzyme-linked receptors found in animals and Recognize various types of signaling molecules
Receptor Tyrosine Kinases
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Signals binding to cell surface are ?first messenger?. Many signal transduction pathways lead to production of second messengers
G-Protein-coupled receptors
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Second messengers relay what?
signals inside the cells
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Examples og second messengers?
cAMP, CA+, and Diacylglycerol (DAG) and inositol triphosphate (IP3)
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Cyclic adenosine monophosphate . Signal binding to GPCR activates G protein to bind GTP, causing dissociation, freeing a subunit. a subunit binds to adenylyl cyclase enzyme, stimulating synthesis of cAMP
Signal transduction via cAMP
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cAMP nactivates what?
Protein Kinase A (PKA)
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PKA does what?
phosphorylate specific cellular proteins.
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PKA targets include?
Enzymes, structural proteins, and transcription factors.
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