what is the purpose of the chopper in an atomic absorption spectrophotometry?
correct for the amount of light emitted by the flame.
which best describes the process of fluorescence?
molecules emit a photon at lower energy when exited electrons return to the ground state
which procedure is not currently or routinely used for point of care testing devices?
Polymerase chain reaction
- Identifying novel proteins as potential new bio markers for disease
- Identifying post transitional modifications of proteins
- understanding the mechanism of disease.
- determining which genes are expressed and which genes are dormant.
all are objectives of?
Proteomics research .
T or F?
mass spectrometers can be used to sequence DNA?
False
what is the driving force for more automation?
High-volume testing
Fast turn around time
Expectation of high quality, accurate results.
Which of the approaches to analyzing automation can use mixing paddles to stir?
Discrete analysis
which type of analyzer offers random access capabilities?
Discrete analyzer
Primary considerations in the selection of an automated chemistry analyzer are?
Total instrument cost
The cost of consumption
The labor component
An example of a modular integrated chemistry/immunoassay analyzer would be?
Dimensions Vista 3000T
Time between initiation of a test and the completion of the analysis is know as ?
Dwell time
What are the advantages of automation?
increased number of tests performed
Minimized labor component
Use of smaller amounts of sample and reagents in comparison to manual procedure.
which steps in automation generally remain a manual process in most labs?
peroration of the sample.
which chemistry analyzer uses slides to contain entire reagent system?
Vitros analyzer
what kind of lamp is used by reflectance spectrometry ?
Tungsten - halogen lab
modification in micro-sampling and reagent dispensing improves what phase of clinical testing?
Analytical.
Bidirectional communication between chemistry analyzer and laboratory information system had the greats impact of which of the phases of clinical testing?
Postanalytical.
Strength of binding between an antigen and antibody is related to ?
Goodness of fit between the epitop and the F(ab) fragment
In monoclonal antibody production the specificity of the antibody is determined by ?
Sensitized B lymphocytes
which unlabeled immune precipitation method in gel is used to quantitate a serum protein?
Immunofixation electrophorises
In immunofixation electrophorises , discrete bands appear at the same electrophoretic location. One reacted with IgA( alpha chain specific) reagent and the other reacted with antihuman λ reagent. This is best described as?
an IgA λ polyclonal protein
In nephelometry, the antigen - antibody complex formation is enhanced in presence of?
Polyethylene glycol.
Which homogeneous immunoassay on inhibiting the activity of the enzyme label when bound to antibody reagent to eliminate separating free-labeled reagent?
EMIT
In flow cytometry, the side scatter is related to?
Granularity of the cell.
The nucleic acid technique in which RNA is converted to cDNA which is then amplified, is known as?
RT-PCR
you analyze the dna content on the sample for breast tissue for suspected malignancy using flow cytometry and gel the following results. DI = 2.5 and % cells in S phase = 29%.
What can you conclude based on this results?
likely indicative of malignant breast tumor.
A CLIA waived test requires that operators?
must follow manufacturer's instructions.
quality control lockout
prevents testing when quality control has not been performed.
EQC per CLIA means?
equivalent
for laboratory with a provider performance microscopy procedure CLIA certificate, which would Not be approved?
Hematology differential.
Connectivity for POCT provides the ability to?
transmit patients results to the medical record
which specimen is NOT used for a waived POC test?
Plasma
for non - waived testing is?
NOT required for physicians in their specialty
Standardization produces?
Decreased work
Improved regulatory compliance
Compatibility of test results between separate locations in the hospital.
Information needed to determine whether POCT should be used include?
who will perform the test
How will the test results be documented
How many tests will be performed per month
which of the positions is not a part of well organized POCT program ?
