A special type of fluorescence microscope that allows you to see objects in 3 dimensions.
Control experiment
Experiment set up to ensure that only one variable is tested when performing an experiment to test a hypothesis.
Deductive reasoning
Forming and testing a hypothesis
“Scientific Method”
Deductive reasoning (“If… then…” logic)
Deductive: General to Specific
Fluorescence microscope
A light microscope that uses light with a shorter wavelength than visible light (UV) to excite dyes that then emit light (fluorescence) at a longer wavelength.
Hypothesis
A possible explanation for an observation that is testable by experiment.
inductive reasoning
Making observations /measurements (data) - quantitative - qualitative - must be repeatable
Inductive: Specific to General
Light microscope
An optical instrument with lenses that refract (bend) visible light to magnify images of specimens.
Magnification
Appearing to make objects look larger such that very small objects such as cells can be seen by the human eye.
prediction
In conducting an experiment you are testing a specific prediction arising from your hypothesis.
Resolving power
A measure of the ability of a lens to discriminate between two objects.
Scanning electron microscope (SEM)
A microscope that uses an electron beam to scan the surface of a sample to study details of its topography.
Scientific Theory
A broad explanation supported by many observations or experiments
Transmission electron microscope (TEM)
A microscope that passes an electron beam through very thin sections, primarily used to study the internal ultrastructure of cells.