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Changes in infected neuts with Hepatozoon in flat headed cats VCP 2008
- gamonts in neuts (1-2%)
- infected neuts: decr. spec granules, incr mito, ANAE and b-glucuronidase +, SBB -
- uninf neuts: SBB+, ANAE and b-gluc -, eos - all neg, baso - SBB-,ANAE and b-gluc+
- lymphs: SBB -, ANAE and b-gluc focal +, monos: only ANAE focal +
- Look at bunch of EM pics of cells and of gamonts in parasitophorous vacuoles
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Immunophenotyping lymphs of cynomolgus monkeys VCP 2008
Stained for CD3 (panT), CD3/CD4 (TH), CD3/CD8 (CTL), CD3-/CD16 (NK), CD3-CD20 (B)
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Rapid agglut for detection of Ca and Aa groups in horses VCP 2008
- Prefered donor: Aa, Qa and Ca NEG
- Most rxns d/t: Aa and Qa
- Most naturally occuring abi are Aa and Ca - both aglu and hem
- Qa - hem
- Aa - severe reactions
- Study showed that rapid blood-typing protocol can be used to detect Aa and Ca abi
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Bcl-2 in feline lymphoma cell lines VCP 2008
- Bcl-2 is antiapoptotic
- Results: incr expression in lymphoma cell lines, decr in peripheral bld monos, but not decr in fresh blood
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IgM interference with Hb using Cell-Dyne VCP 2008
ammonium salt in lysing reagent precip protein causing incr in Hb and MCHC
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Pure WBC aplasia in dog VCP 2007
- Steroid responsive npenia, likely immune mediated, may see concurrent IMHA, ITO, meningitis/polyarthritis
- BM - myeloid hyperplasia (abundant bands, few segs)
- Review this paper
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Heme characteristics of captive westen barred bandicoots VCP 2007
- Hyposeg and annular neuts, monos and occas eos seen
- young had more poly than adults
- see images EM and light micro
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Lead poisoning on swans and Canada geese hematology VCP 2007
- Pb inh heme syn via inh of ALA synthase, ALA dehdrase and ferrochelatase
- PCV, Hb and MCHC dec with lead
- Uric acid and GGT incr in swans
- Phos dec in geese
- Bld smear: incr rubricytes, baso stip, incr poly
- see Hb synthesis in Stokham
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Outbreak of canine monocytic ehrlichiosis in Saudi Arabia VCP 2007
- cellular immune response more NB than humoral but latter may contribute to clinical signs
- low incidence of Tpenia in these dogs (2/9), most had anemia and leukopenia
- Dx: PCR best
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Pelger Huet in Arabian horse VCP 2007
- Both cases in horses have been arabians
- Autosomal dom, heterozygotes - abnormality but no clinical signs, homozyg - lethal
- mutation of B-laminin in people and mice
- Dx: gran with hyposeg, r/o PseudoPH: infection, neoplasia, drugs - sulfonamides, colchicine, chemo, document vertical transmission
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M7 leuk in a dog CD34+ CD41+ VCP 2007
- CD41+ GPIIb, CD61 GP IIIa
- CD42 GPIb (more mature plt assoc marker)
- Cyt expression of CD41 and 61 more spec and se d/t possible adherence of plts to surface of blasts
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Heme of young pretraning greyhounds VCP 2007
- RBC, HCT, Hb pos correlation with age
- Plt count neg correlation with age
- No sex diff
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Plt fxn in dogs, breed differences and effect of ASA admin VCP 2007
- higher plt ag in CKCS compared with other breeds
- longer CT in cairn ter compated to boxers
- ASA prolonged CT (col+epi but not col+ADP) and decr plt ag
- So... Col+Epi CT dependent on TxA2 since it is inh by ASA
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Spirochetemia by Borrelia turicatae in 3 dogs VCP 2007
- Heme: anemia, neutrophilia, lymph/eos/Tpenia
- rodent natural reservoir
- more likely to be spirochetemic than B. burgdorferi but abi xreact
- use PCR for flagellin to differentiate
- see pics - looks like mine
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Bld smear from preg cat after abortion: Cytauxzoon felis VCP 2007
- Bobcats are reservoir
- Schizonts form in macs, merozoites infect RBCs, see signet ring piroplasms within RBCs or schizont laden macs
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Glanzmann thrombasthenia in Oldenborg filly VCP 2007
- Prolonged bleeding time, normal plt count, normal coags and vWF
- Decr plt ag to ADP, col, thrombin or epi, wk ag to ristocetin
- TEG: K increased, alpha angle dec and MA decr (80% MA dependent on function and #)
- GPIIb subunit mutation - absence of plt CD41 and decr CD41/CD61
- Binds fibr with high affinity, so plts don't adhere to each other or to underlying ECM (other prot can bind GPIIb/IIIa
- such as fibronectin, col, vibronectin and vWF)
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Heme and biochem of persian squirrels VCP 2007
hyperseg neuts common, no basos or band seen, Hb migraded ahead of human Hb A
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Performance of 2 microtiter Coombs tests in dog VCP 2007
- VMRD uses single polyvalent antiserum toward canine IgG, M, C3
- U of MN test uses both polyvalent and monovalent antisera
- U of MN test more se, VMRD more sp use of multiple antisera dilutions incr se, monovalent antisera did not incr se
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Coag markers in healthy cats and cats with subclinical HCM VCP 2007
- measured TAT, Ddimer, PT, aPTT - no sign between groups
- 45% of cats with HCM had evidence of hypercoag, TAT was most se (25% of cats with HCM was incr), followed by Ddimer and FDPs
- Ddimer may not be a se marker in cats of activated coag and fibrinolysis
- LA size not associated with hypercoag
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Eval of CA530-VET heme analyzer for vet practices VCP 2007
impedence analyzer, not accurate for plts, feline WBC when normal or low, diffs exept neuts
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Plt parameters in citrate vs EDTA bld VCP 2007
- Plt count, MPV and MPC all lower with citrated blood, d/t plt activation and agregation, MPV and MPC inaccurate
- d/t inadequate sphering
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