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MCB Lec 2
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Resolution
the ability to distinguish closely positioned objects as separate images
Resolving power of human eye
0.2 mm
resolving power of LM
0.2 microm
resolving power of TEM
1 nm
Resolving power of SEM
2.5 nm
General Histological Approach
1. Fix
2. Infiltrate and embed
3. Stain
Acid Dye
Organic or inorganic compound that carries a permanent negative charge
Basic Dye
Organic or Inorganic compound with a permanent positive charge
Enzyme Histochemistry
the location of an enzyme is determined by taking advantage of its catalytic activity
Schiff Reagent
PAS method to localize carbohydrates
Fuelgen reaction to stain DNA
Immunocytochemistry
antibodies used to locate specific molecules (antigen)
Autoradiography
localization of radiolabeled molecules
In Situ Hybridization
used to detect RNA or DNA using nucleic acid probes to locate complementary nucleotide sequences
Author
caitlin
ID
30116
Card Set
MCB Lec 2
Description
Methods to determine cell, tissue, and organ structure
Updated
2010-08-16T23:45:30Z
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