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Variation in size of erythrocytes?
Anisocytosis
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Lymphocyte that occurs in response to viral infection and that is common in infectious mononucleosis; Reactive lymphocyte ?
Atypical lymphocyte
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a determination of the relative numbers of each type of white blood cell when a specified number (usually 100) is counted; white blood cell differential count; leukocyte differential count
Differential count
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having reduced color or hemoglobin content
Hypochromic
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having a larger-than-normal cell size
macrocytic
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having a smaller-than-normal cell size
microcytic
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having normal color
Normochromic
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having a normal cell size
Normocytic
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the engulfing of a foreign particle or cell by another cell
phagocytosis
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significant variation in the shape of red blood cells
poikilocytosis
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see atypical lymphocyte
reactive lymphocyte
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What is the purpose of a WBC differential count?
- determine the percentage of each type of WBC in peripheral blood smear
- make observations on RBC morphology
- estimate platelet number
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Describe the area of the smear in which the differential count should be performed.
area near the feathered edge of the smear where RBC are barely touching each other.
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What three characteristics of WBCs are evaluated in cell identification?
- Cell size
- Nucleus: shape and structure
- Cytoplasm: amount, color, inclusions
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what is the percentage of segmented neutrophils?
- 1m/o: 15-35
- 6 and 12 y.o.: 45-50
- adult: 50-65
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what is the percentage of band neutrophils?
- 1m.o: 7-13
- 6 y.o and Adult: 0-7
- 12 y.o.: 6-8
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what is the percentage of Eosinophils?
1m/o; 6y.o.; 12 y.o.; and adult: 1-3
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what is the percentage of Basophils?
1m/o; 6y.o.; 12 y.o.; and adult: 0-1
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Monocyte percentage?
- 1m/o: 5-8
- 6y.o.: 4-8
- 12 y.o: 3-8
- adult: 3-9
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Lymphocyte percentage?
- 1m/o:40-70
- 6y.o.:40-45
- 12 y.o.: 35-40
- adult: 25-40
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What are the functions of neutrophils?
- active in phagocytosis
- increased during bacterial infections
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What are the functions of monocytes?
primary defense against invading pathogenic organisms
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What are the functions of lymphocytes?
- important in antibody production
- communicating with other cells in immune system
- production of cytokines.
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conditions that affect WBC percentage?
- Bacterial infection
- Viral infection
- infectious mononucleosis
- parasitic infection
- allergic reactions
- Leukemias
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Increased total WBCs, increased percentage of neutrophils andbands
Bacterial infection
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Decreased WBCs, increased percentage of lymphocytes
Viral infection
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Increased total WBCs,
increased lymphocytes,
increased atypical lymphocytes
Infectious mononucleosis
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Increased eosinophils
- parasitic infections
- allergic reaction
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Total WBCs usually increased
increase in type of leukocyte involved
Leukemias
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RBC morphology during Iron deficiency anemia,thalassemias
Microcytic
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RBC morphology in case of Vitamin B12 deficiency, folate deficiency?
Macrocytic
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RBC cell morphology in case of Hereditary spherocytosis
Spherical
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RBC hemoglobin content in case of Iron deficiency anemia and Spherocytosis is
Hypochromic
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