An organism that does not use molecular oxygen (02) but is not affected by its presence
Agar
A complex polysaccharide derived from a marine alga and used as a solidifying agent in culture media
Bacterial Growth Curve
A graph indicating the growth of a bacterial population over time
Binary Fission
Prokaryotic cell reproduction by division into two daughter cells
Budding
(1) Asexual reproduction beginning as a protuberance from a parent cell that grows to become a daughter cell. (2) Release of an enveloped virus through the plasma membrane of an animal cell
Catalase
An enzyme that catalyzes the breakdown of hydrogen peroxide to water and oxygen
Chemically Defined Medium
A culture medium in which the exact chemical composition is known
Colony
A visible mass of microbial cells arising from one cell or from a group of the same microbes
Culture
Microorganisms that grow and multiply in a container of culture medium
Culture Medium
The nutrient material prepared for growth of microorganisms in a laboratory
Death Phase
The period of logarithmic decrease in a bacterial population; also called logarithmic decline phase
Direct Microscopic Count
Enumeration of cells by observation through a microscope
Enrichment Culture
A culture medium used for preliminary isolation that favors the growth of a particular microorganism
Extreme Halophile
An organism that requires a high salt concentration for growth
Extreme Thermophile
An organism whose optimum growth temperature is at least 80 c; also called extreme thermophile
Facultative Anaerobe
An organism that can grow with or without molecule oxygen (02)
Facultative Halophile
An organism capable of growth in, but not requiring, 1-2% salt
Filtration
The passage of a liquid or gas through a screenlike material; a 0.45 -um filter removes most bacteria
Generation Time
The time required for a cell or population to double in number
Hydroxyl Radical
A toxic form of oxygen (OH*) formed in cytoplasm by ionizing radiation and aerobic respiration
Hyperthermophile
An organism whose optimum growth temperature is at least 80 C; also called extreme thermophile
Inoculum
A culture medium in which microorganisms are implanted
Lag Phase
The time interval in a bacterial growth curve during which there is no growth
Log Phase
The period of bacterial growth or logarithmic increase in cell numbers; also called exponential growth phase
Maximum Growth Temperature
The highest temperature at which a species can grow
Mesophile
An organism that grows between about 10 C and 50 C; a moderate-temperature-loving microbe
Microaerophile
An organism that grows best in an environment with less molecular oxygen (O2) than is normally found in air
Most Probable Number (MPN) Method
A statistical determination of the number of coliforms per 100 ml of water or 100 g of food
Nutrient Agar
Nutrient broth containing agar
Nutrient Broth
A complex medium made of beef extract and peptone
Obligate Aerobe
An organism that requires molecular oxygen (O2) to live
Obligate Anaerobe
An organism that does not use molecular oxygen (O2) and is killed in the presence of O2
Obligate Halophile
An organism that requires high osmotic pressures such as high concentrations of NaCl
Optimum Growth Temperature
The temperature at which a species grows best
Organic Growth Factor
An essential organic compound that an organism is unable to synthesize
Peroxidase
An enzyme that breaks down hydrogen peroxide
Peroxide Anion
An oxygen anion consisting of two atoms of oxygen (02 2-)
Plasmolysis
Loss of water from a cell in a hypertonic enviroment
Plate Count
A method of determining the number of bacteria in a sample by counting the number of colony-forming units on a solid culture medium
Pour Plate Method
A method ofinoculating a solid nutrient medium by mixing bacteria in the melted medium and pouring the medium into a Petri dish to solidify
Psychrophile
An organism that grows best at about 15 C and does not grow above 20 C; a cold-loving microbe
Serial Dilution
The process of diluting a sample several times
Singlet Oxygen
Highly reactive molecular oxygen (O2 -)
Spread Plate Method
A plate count method in which inoculum is spread over the surface of a solid culture medium
Stationary Phase
The period in a bacterial growth curve when the number of cells dividing equals the number dying
Sterile
Free of microorganisms
Streak Plate Method
A method of isolating a culture by spreading microorganisms over the surface of a solid culture medium
Superoxide Dismutase (SOD)
An enzyme that destroys superoxide free radicals
Superoxide Free Radical
A toxic form of oxygen (O2 -) formed during aerobic respiration
Symbiosis
The living together of two different organisms or populations
Thermophile
An organism whose optimum growth temperature is between 50 C and 60 C; a heat-loving microbe
Trace Element
A chemical element required in small amounts for growth