immuno 008 antibodies part 2 (C-regions V-regions H-chain L-chain immunoglobulin domains epitop

aromatic amino acids (Phe, Tyr, Trp) which can form strong hydrophobic interactions

it is the first antibody produced in response to a pathogen. By isotype switching, synthesis of IgM gives way to synthesis of IgG

it's the most abundant antibody. IgG is smaller and more flexible than IgM. During pregnancy, it can be transferred across the placenta to provide the fetus with protective antibodies from the mother

a monoclonal antibody neutralizing TNF; used in rheumatoid arthritis.

binds protein (HER2) expressed on some tumor cells (breast cancers, lymphomas); inhibits cancer cell proliferation

ELISA, Western Blotting, Immunofluorescence, immunoprecipitation

• 1. proteins separated by electrophoresis on a protein SDS-PAGE.
• 2. proteins transferred to (nitrocellulose or PVDF) membrane sheets.
• 3. protein bands visualized with enzyme tagged antibodies