Culture Media

this type of media grows most non-fastidious organisms with no advantage given to a particular organism.

this type of media is enriched for fastidious organisms.

this type of media is inhubitory.

this type of media is used as a visual aid.

this type of media is confirmatory ID for previously isolated organisms.

this is an examle of a media that is supportive and also differential

this is an example of a media that is selective and also differential

this is the process of taking bacteria from a site and growing it in a culture.

these two bacteria cannot be grown in vitro.

what three things are needed to sustain microbial metabolism?

this type of water is recommended to avoid impurities in re-hydrated powder media.

this is the original solidifying agent.

this is the most common solidifying agent

agarose dissolves at what temperature?

agarose re-solidifies at what temperature?

how much agarose should be used in solid media?

how much agarose should be used in semi-solid media?

how much agarose should be used in liquid media?

in a THIO broth what is the purpose of agarose?

there are partially digested protiens which function as the principal source of organic nitrogen.

this is a peptone used in basic media

this is the preferred type of whole blood because it has the correct hemolytic reaction

human blood is limited for the use of what organism?

what are the two selective media that horse blood is limited to?

why is hemolyzed blood used?

what are the three meat and tissue infusions that are used in media?

this provides a carbohydrate additive and a carbon source

this helps to maintain the isotonic or physiological pH of the cell wall

at what percentage is sodium chloide inhibitory

this helps to maintain H ion concentration.

this is used as an endpoint to ID organisms.

these help to visually detect pH changes based on bacterial action

what are the colors for phenol red colormetric indicator?

what are the colors for methyl red colormetric indicators?

what are the colors for the neutral red indicator?

what are the colors for the bromcresol purple colormetric indicator?

what are the colors for the ortho-toluidine blue colormetric indicator?

what are the colors for the bromthymol blue colormetric indicator?

what is the sulfur source for the formation of H₂S gas?

what is the positive endpoint of an H₂S indicator?

this promote growth of certain groups of organisms

this retards growth of certain groups of organisms

these two terms regarding culture growth are used interchangeably

what three dyes are used as GP inhibitors?

what is the dye used in the MAC plate?

what is the dye used in the SS and BS plates?

what organisms are inhibited by brilliant green?

this is used in enteric media to inhibit GNC and GP organisms.

what percent of sodium chloride is inhibitory for all organisms except streptococcus and staphylococcus?

what percent of sodium chloride is inhibitory for all organisms excep staphylococcus?

this inhibits all organisms except salmonella and vibrio

what antibiotic is used to inhibit garm positive organisms?

what two antibiotics are used to inhibit gram negative organism?

what antibiotic is used to inhibit yeast

this media has peptones and is used in susceptibility testing for both fastidious and nonfastidious organisms.

this media has peptones and glucose and is used to speed bacterial growth rate.

This media has beef infusion and starch and used for susceptibility testing and mainttenance.

this media has calf brain, beef heart, peptones and glucose, and is used for quality control.

this media is used to accelerate growth to log rate in 6-8 hours. It is the blood culture media base.

this media contains thioglycolic acid and is used to prevent the formation oand accumulation of toxic H₂O_2.

in THIO pink coloration seen in the uppermost portion of the medium is caused by what?

what is the O₂ indicator in Thioglycollate Medium?

this is the most frequently used broth in micro.

what are three indications for THIO?

this media consists of TSA, brucella, BHI, and is enriched with 5% defibrinated sheeps blood.

this plate is used in the differentiation of hemolysis

this is partial hemolysis, green zone visible due to presence of biliverdin.

this is complete hemolysis, there is a visible clear zone.

this is when there is no hemolysis

how are hemolytic reactions enhanced in Blood Ager Media?

streptolysin-O is ______ labile.

this plate has 2% hemoglobin or isovitalex and 5% hemolyzed sheeps RBC's

this plate cultivates fastidious organisms needing NAD/Hemin within RBC (Neisseria, Haemophilus).

this media is selective for N. gonorrhoeae and N. meningitidis.

this media is more selective than TM when working with mixed flora.

what is added to TM plates to inhibit proteus?

this is selective for N. Gonorrhoeae only.

the constituents of this plate is peptones, cornstarch, 3% HgB, horse plasma, and antibiotics

what three antibiotics are added to Thayer Martin media?

This is a gram positive selective media that has 7.5% NaCl.

what is the pH indicator in MSA?

what media is used as selective for staphylococcus?

this media is 95% positive for S. aureus.

This media is enriched with 5% sheeps blood and selective for GP organisms

this media separates GP organisms from swarming enteric bacteria (e.g. proteus).

what is the biochemical agent in MAC?

what is the pH indicator in MAC?

what are the two inhibitors in MAC?

on an MAC plate strong lactose fermenters leave a zone of percipitated bile that turns what color?

MacConkey Agar is selective for what organisms?

this media is enriched with a yeast extract and is used for the recovery of salmonella and shigella from stool samples.

what are the two pH indicators in HE?

what is the H₂S indicator in Hektoen-Enteric Agar?

what inhibitor is used in Hektoen-Enteric Agar?

HE is selective for Gram _______ organisms.

what colors are fermenters on an HE plate?

what color do nonfermenters turn on an HE plate?

on an HE plate strong fermenters leave a zone of percipitated bile that is what color?

what is the pH indicator on the salmonella-shigella Agar?

what biochemical agent do MAC, HE, and SS share?

what is the H₂S indicator on Salmonella-Shigella Agar?

what are the three inhibitors on the Salmonella-Shigella Agar?

what is the H₂S indicator on Xylose-Lysine-Desoxycholate Agar (XLD)?

this media is used for the isolation of salmonella and shigella from other GNR.

what color are fermenters and nonfermenters on XLD?

this broth temporarily inhibits normal flora for the isolation of salmonella and shigella.

after 6-8 hours of GN broth sample should be subcultured to what three media?

gram negative enteric media differentiates what?

this semi-solid media differentiates by utilizing or not utilizing carbohydrates.

What is the pH indicator on O-F?

what are the steps to inoculate O-F tubes?

in an O-F tube what interpretation is based on the production of what?

in O-F if acid is produced ony in the open tube the result is:

in O-F if acid is produced in both tubes the result is:

in O-F if acid is produced in neither tube the result is:

this is a semi-solid medium with a cystine biochemical that is used to differentiate neisseria species.

what is the pH indicator in CTA?

this agar has a biochemical of deoxyribonucleic acid at 0.2%.

what is the indicator in Dnase agar?

the purpose of this media is to detect bacterial DNase production

Dnase agar is ___ positive for S. aureus.

DNase is ___ positive for S. marcescens.

what is the appearance of a positive result on DNase?

what is the media protocol for throat cultures?

what is the media protocol for nasopharyngeal cultures?

what is the media protocol for sputum?

what is the media protocol for urine?

what is the media protocol for wound cultures?

what is the media protocol for stool?

what is the media protocol for genital cultures?

what is the media protocol for CSF?

what size glassware is used to prepare media?

unprepared media is extremely __________.

small loads should be sterilized at what temp? for how long?

large loads should be sterilized at what temp? for how long?

at what temperature do you add blood to the mdium for CHOC?

at what temperature do you add enrichments including whole blood?

at what temperature should the medium be poured into a petri dish?

how do you eliminate bubbles in a medium?

how do you eliminate condensation on a petri dish?

how much medium should be used to achieve a 3mm depth in a 100 mm petri dish?

how much medium should be used to achieve a 5-6mm depth in a 150mm petri dish?

what is the shelf life of media?

what type f media has a longer shelf life?

what percent of prepared plates should be tested for quality control?

what is the ideal temperature for incuabtion?