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Amplification (i.e. making lots of copies!) of DNA is accomplished by what process?
polymerase chain reaction
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These match: cDNA and ____
Synthesized using reverse transcriptase
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These match: mutagens and _____
create changes in microbes' genomes to change phenotypes
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These match: Synthetic DNA and _____
creation of genes for specific proteins
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In gel electrophoresis, DNA molecules move toward the ___ electrode because they have an overall ____ charge.
positive / negative
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What would NOT be used to sterilize a mattress?
filtration, ionizing radiation
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What would be used to sterilize a mattress?
ethylene oxide
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The individual dairy creamers used in restaurants is NOT usually treated by what?
filtration, ionizing radiation, autoclaving
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The individual dairy creamers used in restaurants is usually treated by what?
ultra-high temperature pasteurization
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What statements are false about disinfectants?
effective in destroying endospores; used for sterilization; used on living tissue
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This statement is True about disinfectants: They are used on ____ surfaces.
inanimate
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What is the process of incineration using the bacterincinerators NOT used for?
degerming, disinfection, or sanitization
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What is the process of incineration using the bacterincinerators in lab used for?
sterilization
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What is not the target of antimicrobial agents?
cytoplasm
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What are the targets of antimicrobial agents?
cell walls, cell membranes, ribosomes, DNA
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What physical agent is not included for controlling microbial growth?
sodium hypochlorite
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What are the physical agents for controlling microbial growth?
UV radiation, boiling water, HEPA filter
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What is the destruction of all microbes in or on an object called?
sterilization
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What is the reduction of potential pathogens on living tissue?
antisepsis
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What is the destruction of most pathogens on nonliving surfaces?
disinfection
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What is the removal of pathogens to meet public health standards?
sanitation
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Name the process to kill pathogens and reduce spoilage microbes in beverages.
pasteurization
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Modification of ____ _____ is usually considered an ethical, legal, or social issue (ELSI) regarding recombinant DNA technology.
crop plants
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The screening of humans for what is usually considered an ethical, legal, or social issue (ELSI) regarding recombinant DNA technology?
genes that predispose them to diseases
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Dr. Kary Mullis won the Nobel Prize for Chemistry in 1993 for his invention of this technique used extensively in biotechnology.
PCR (polymerase chain reaction)
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What is usually not considered an ethical, legal, or social issue (ELSI) regarding recombinant DNA technology?
answering basic research questions
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What can DNA fingerprinting NOT be used for?
to generate cDNA clones; to generate DNA libraries; to make subunit vaccines like Hep B
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What is usually considered an ethical, legal, or social issue (ELSI) regarding recombinant DNA technology?
modification of animals to produce pharmaceuticals and spare organs for humans
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What can DNA fingerprinting be used in?
forensic investigations
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Amplification (i.e. making lots of copies!) of DNA is NOT accomplished by:
gene probes, DNA sequencing, or western blot
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