Uses two beams of ligt separated by prisms to prodce a 3D brightly colored image of living microbes
Differential Interference Contrast Microscopy
Uses a condenser with ring shaped diaphram and diffraction plate; brings direct and diffracted light waves together to produce images; used to observe detailed internal structures of unstained living organisms
phase-contrast microscopy
Scanning
Low Power
High Power
Oil Immersion
4X
10X
40X
100X
Basic Stains
Possitive Charged (Cations)
Bind to negatively charged cell structures
Acidic Stains
Anions Negatively charged, bind to possitively charged structures
Used in negative staining
Bacteria
Prokaryotic
Binary Fission
Beta Lycam Coat
Escherichia coli
Archea
prokaryotic
lack Beta Lycam
Thermus aquaticus
Fungi
eukaryotes, absorb nutrients from enviornment
nonmotile
Murcor
Protozoa
Unicellular eukaryotes, get food from engulfing other organisms.