BIO LAB FINAL

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Bacteria are too small to separate...

directly
indirect separation it used by what...

serial dilution
CFU equation

number of colony/ (dilution X amount plated)
Effect go pH equation

-log(H+)
pH can lead to..

cell death
pH organisms. 3
- Acidophiles: lower than 5.5
- Nuetrolphiles: 5.5-8.5
- Alkaliphiles: greater than 8.5
Importance of osmotic pressure is...

maintain equilibrium.. not too much water going in or out
osmosis def

water movement from where its concentration is high to where it is low
osmotic pressure organisms 3
- hyposmotic: h20 move into cell
- hyperosmotic: h20 moves out of cell
- isosmotin: h20 moves in both directions
Countable plate for CFU

25-300
Salt osmo pressure bacteria 3
- halophilies: 3% high NaCl
- Extreme halophiles: 15-25% NaCl
- Osmotolerant: grow on range
temperature bacteria 5
- psychrophiles: <20C
- Psychotrophs: 0-30C
- mesophiles: 15-45C
- thermophiles: >40C
- Extreme thermophiles: 65-110C
UV light def

type of electromagnetic energy
what type of UV light is most harmful

UV-C
UV causes new _____ to be formed in bacteria DNA. Most common one.
- Pyrimidine dimers
- thymine dumers
Phenylethyl Alcohol Agar (PEA) is what type of medium and isolates what bacteria
*think Positive
- undefined and selective (inhibits growth of gram negative)
- staphylococci, streptococcie, enterococci
Mannitol Salt Agar (MSA) is what type of medium and isolates what bacteria
- Selective and differential. Only G + can grow.
- differentiates between staphylococcus species
on the MSB (mannitol salt Agar) what does yellow with halo, pink and no growth mean.
- Y: positive mannitol salt fermentation
- P;no fermentation
- NG: probably gram negative

Author

berraL13

270001

Card Set

BIO LAB FINAL

Description

Micro Bio Lab Final WMU

Updated

2014-04-10T20:37:40Z

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