What are the advantages and disadvantages of UV VIS?
Advantages: Relatively simple to operate
Disadvantages: not suitable for very low concentrations
How does chromatography work?
Components of a structure is separated based on their adsorption chracteristics
In chromatography, there is a stationary phase which is the solid that the components adsorb to and desorb from. This in the column in which the components travel through.
To aid it move through the column, there is a mobile phase, which is what carries and pushes the components along the stationary phase.
How does atomic absorption spectroscopy work?
The analyte is vapourised by placing it in a flame and a wavelength specifically able to have enough energy to excite electrons from the lower energy level to the higher energy level is passed through the analyte
The analyte absorbs some of the radiation. The light beam is then passed through a monochromator to select the light of chosen wavelength and its intensity is measured by a detector
How does UV VIS work?
A specific wavelength that the substance will absorb is used and passed through the analyte and the amount that passes through is measured by a detector
When a substance absorbs visible light, it appears coloured. This is the complementary colour