Microbiology review

  1. Do work
  2. Regarding early life on Earth,
    microbial life existed for billions of years before plant and animal life.
  3. In what/which domain(s) of life is/are microorganisms not represented
    e.  none of the above
  4. Marie Petri and Fannie Hesse are credited with giving ________ the idea to use agar
    as a solidifying agent and to use semi-transparent closed lid plates for
    bacterial cultivation.
    Robert Koch
  5. a
    a.  miasma; germ theory of disease
  6. Chemolithotrophy involves
    oxidation of inorganic compounds.
  7. The ultimate limit of what we are able to see with a microscope is dictated by
  8. Which of these statements is TRUE?
    • The gram-positive wall contains a thick
    • peptidoglycan layer but is less complex than the gram-negative wall.
  9. A transmission electron microscope (TEM) is generally used _______, whereas a scanning electron microscope (SEM) is more commonly
    used _______.
    • to observe intracellular structures of thin
    • sections / to observe surface structures
  10. _________________ developed the concept of the “magic bullet” a key conceptual idea in the
    understanding of antibodies and the __________________ of antibiotics.
    Paul Ehrlich; selective toxicity
  11. Although not given his due credit, _________________ designed a fairly conclusive experiment that disproved the theory of spontaneous generation almost 200 years before Pasteur’s swan necked flask experiment
  12. Which of the following is not one of Koch’s postulates?
    isolate its DNA and identify organism by DNA sequence
  13. ________ accelerates the natural "clean up" process by introducing pollutant-consuming microorganisms or specific nutrients that help
    microorganisms degrade pollutants
  14. Bacterial cells can only sense a gradient by identifying ____________ changes rather than ____________ differences due to their small ___________.
    temporal / spatial / cell size
  15. A glass capillary tube containing a chemical was placed inside a turbid axenic culture of cells. Most cells moved away from the tube, but some cells were found inside the capillary tube. The chemical is likely a(n)
    repellent to all cells and some cells by chance were within the tube due to Brownian motion.
  16. The prokaryotic transport system that involves a substrate-binding protein, a membrane-integrated transporter, and an ATP-hydrolyzing protein is
    the ABC system
  17. When does endospore formation commence?
    when bacterial growth ceases due to limitation of an essential nutrient
  18. A general term to describe intracellular accumulations of polyphosphate, elemental sulfur, or magnetosomes is a(n) ________.
  19. Which statement is TRUE?
    Peritrichous flagella are all over the bacterial cells; lophotrichous flagella are tufts on the "ends" of bacterial cells.
  20. A microbiologist inoculates Staphylococcus aureus into a culture medium. Following incubation, both Staphylococcus aureus and Staphylococcus epidermidis are determined to be growing in this culture. What is the most likely explanation?
    the culture is contaminated
  21. Siderophores
    bind iron and transport it into the cell.
  22. How is the ΔG0' of a reaction calculated?
    add the Gf0 of all the products; then subtract the Gf0 of all the reactants
  23. MacConkey Agar contains bile salts that inhibit the growth of Gram positive organisms. In addition this particular medium is supplemented with the sugar lactose and a pH indicator. MacConkey Agar is therefore considered a ______________ medium.
    a. selective   and    b. differential
  24. By convention, redox reactions are written _______ and the second compound in a redox pair is the _______ form. By convention, redox reactions are written _______ and the second compound in a redox pair is the _______ form.
    as half reactions / reduced
  25. Production of ATP using oxidative phosphorylation occurs when
    the proton motive force was generated from respiring reactions.
  26. Which structure is involved in the genetic exchange (conjugation) between cells?
    ATP; the fermentation products are waste products.
  27. The periplasm is a(n)
    region between the cytoplasmic membrane and the outer membrane of gram-negative Bacteria.
Card Set
Microbiology review
Micro review