micro chapter 3

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  1. what are the 6 I's of culturing microbes
    inoculations, isolation, incubation, inspection, information gathering, and indentification
  2. what is the introduction of a sample into a container of media to produce a culture of observable growth
  3. what is the separating of one specimen from another
  4. what is the phase where microbes are under the conditions that allow growth
  5. what is a mound of cells that consists of only one type of species
  6. what are the three isolation techniques
    streak plate, pour plate, and spread plate
  7. during inspection, if a single species is growing in the container it is called
    pure culutre
  8. during inspection, if there are multiple species growing in a container it is called
    mixed culture
  9. during inspection, if there is unknown or unwanted microbes in the culture it is called
  10. what is being identified in cell and colony morphology or staining characteristics
  11. what is being identified by the DNA sequence
  12. what is being identified by biochemical tests to determine an organism's chemical and metabolic characteristics
  13. what is being identified by immunological tests
  14. what are the three properties that media can be identified
    physical state, chemical composition, and functional type
  15. what classification of media can be liquid, semisolid and solid
    physical state
  16. what classification of media uses synthetic and complex means
    chemical compostition
  17. what classification of media has general purpose, enriched, differential, anaerobic, transport, assay, and enumeration
    functional type
  18. what are the three physical states of medai
    liquid, semisolid, and solid
  19. what physical state of media is broth, does not solidify
  20. what physical state of media contains solidifying agents
  21. what physical state of media has firm surface for colony formation, contains solidfying agents; liquefiable and nonliquefiable
  22. what is the most commonly used solidifying agent
  23. what is solid at room temperature, liquefies at boiling, does not re-solidify until cools at 42C
  24. what provided framework to hold moisture and nutrients together
  25. what is not digestible for most microbes
  26. what most commonly used media is liquid medium containing beef extract and peptone
    nutrient broth
  27. what most commonly used media is solid media containing beef extract, peptone, and agar
    nutrient agar
  28. what chemical content of media contains pure organic and inorganic compounds in an exact chemical formula
  29. what chemical content of media contains at least one ingredient that is not chemically definable
    complex or nonsynthetic
  30. what chemical content of media grows a broad range of microbes, usually nonsynthetic
    general purpose media
  31. what chemical content of media contains complex organic substances such as blood, serum, hemoglobin or special growth factors required by fastidious microbes
    enriched media
  32. what media contains one or more agents that inhibit growth of some microbes and encourage growth of the desired microbes
    selective media
  33. what media allows growth of several types of microbes and displays visible differences among those microbes
    differential media
  34. what miscellaneous media contains a substance that absorbs oxygen or slows penetration of oxygen into medium, used for growing anaerobic bacteria
    reducing medium
  35. what miscellaneous media contains sugars that can be fermented, converted to acids, and a pH indicator to show this reaction
    carbohydrate fermentation medium
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micro chapter 3
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