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lcunrod7
on FreezingBlue Flashcards.
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what are the 6 I's of culturing microbes
inoculations, isolation, incubation, inspection, information gathering, and indentification
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what is the introduction of a sample into a container of media to produce a culture of observable growth
incoluation
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what is the separating of one specimen from another
isolation
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what is the phase where microbes are under the conditions that allow growth
incubation
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what is a mound of cells that consists of only one type of species
colony
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what are the three isolation techniques
streak plate, pour plate, and spread plate
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during inspection, if a single species is growing in the container it is called
pure culutre
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during inspection, if there are multiple species growing in a container it is called
mixed culture
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during inspection, if there is unknown or unwanted microbes in the culture it is called
contaminents
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what is being identified in cell and colony morphology or staining characteristics
microbe
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what is being identified by the DNA sequence
microbe
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what is being identified by biochemical tests to determine an organism's chemical and metabolic characteristics
mircrobe
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what is being identified by immunological tests
microbe
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what are the three properties that media can be identified
physical state, chemical composition, and functional type
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what classification of media can be liquid, semisolid and solid
physical state
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what classification of media uses synthetic and complex means
chemical compostition
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what classification of media has general purpose, enriched, differential, anaerobic, transport, assay, and enumeration
functional type
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what are the three physical states of medai
liquid, semisolid, and solid
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what physical state of media is broth, does not solidify
liquid
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what physical state of media contains solidifying agents
semisolid
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what physical state of media has firm surface for colony formation, contains solidfying agents; liquefiable and nonliquefiable
solid
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what is the most commonly used solidifying agent
agar
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what is solid at room temperature, liquefies at boiling, does not re-solidify until cools at 42C
agar
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what provided framework to hold moisture and nutrients together
agar
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what is not digestible for most microbes
agar
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what most commonly used media is liquid medium containing beef extract and peptone
nutrient broth
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what most commonly used media is solid media containing beef extract, peptone, and agar
nutrient agar
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what chemical content of media contains pure organic and inorganic compounds in an exact chemical formula
synthetic
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what chemical content of media contains at least one ingredient that is not chemically definable
complex or nonsynthetic
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what chemical content of media grows a broad range of microbes, usually nonsynthetic
general purpose media
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what chemical content of media contains complex organic substances such as blood, serum, hemoglobin or special growth factors required by fastidious microbes
enriched media
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what media contains one or more agents that inhibit growth of some microbes and encourage growth of the desired microbes
selective media
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what media allows growth of several types of microbes and displays visible differences among those microbes
differential media
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what miscellaneous media contains a substance that absorbs oxygen or slows penetration of oxygen into medium, used for growing anaerobic bacteria
reducing medium
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what miscellaneous media contains sugars that can be fermented, converted to acids, and a pH indicator to show this reaction
carbohydrate fermentation medium
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