cell bio exam III key

  1. the following DNA nuclease produces straight, blunt ended cuts:



    C. HaeIII
  2. dissociation of dsDNA into single strands:




    A. (b or c)
  3. DNA from different individuals varies in:




    B. (a and c)
  4. leaky mitochondrial membranes:


    C. result in cell death
  5. cell membranes from the following organelles are exposed to the external environment:



    D. none of the above
  6. phosphatidylcholine contains:



    A. a quaternary ammonium ion
  7. phosphatidylserine contains:



    C. terminal amino acid and b. an ammonium ion
  8. K+ ion concentrations:



    A. are 30 times higher inside the cell than outside
  9. the passive transporter for glucose:



    A. works with the concentration gradient
  10. the maximum resting membrane potential for an animal cell is approximately:



    A. -0.2 V
  11. the following dye is used to visualize DNA bands after separation on agarose gels.



    A. ethidium bromide
  12. the polymerase chain reaction requires:



    D. all of the above
  13. after 3 cycles of PCR, the number of dsDNA strands resulting from one initial dsDNA strand is:



    B. 8
  14. reagents necessary to produce a recombinant DNA construct for use in E. coli include:




    E. all of the above
  15. from what is cDNA made?



    C. mRNA
  16. the restriction nuclease HindIII produces:
    a. straight blunt ended cuts
    b. staggered cuts with "sticky ends"
    b. staggered cuts with "sticky" ends
  17. DNA can be quantitated by measuring absorbance at _______nm:



    C. 260
  18. Taq polymerase is obtained from the organism:



    A. thermus aquaticus and c. bacillus thermoaquaticus
  19. eucaryotic systems for recombinant gene expression include:



    B. (a and b)
  20. patterns of gene expression in different cell types can be determined by:


    C. replacing genes of interest with reporter genes
  21. genetic manipulations include:



    D. all of the above
  22. a convenient source of natural membranes for experimentation is:


    B. red blood cells
  23. membrane scaffolding proteins include:




    E. (b and c)
  24. integral membrane proteins can be extracted with:



    D. (a and c)
  25. bacterial rhodopsin uses _________ to absorb light energy.



    C. retinal
  26. cells can complex glycolipids:



    A. as recognition motifs
  27. Concanavalin A is a(n):



    C. lectin
  28. Membrane channels are selectively permeable due to:


    B. (a and b)
  29. Ions with hydration shells can be transported by channels:
    a. true
    b. false
    b. false
  30. voltage gated Ca2+ channels:


    B. stimulate neurotransmitter release
  31. membrane channel control mechanisms include:


    B. (a and b)
  32. The nernst equation for membrane potenial is:



    A. V = 62 X log10(co/ci)
  33. the resting membrane potential is dependent primarily on _______ on both sides of the membrane. 



    C. [K+]
  34. the primary advantage of cDNA is:



    B. lack of introns
  35. restriction mapping of genomal segments:



    C. (b and c)
  36. Microarrays:




    D. (b and d)
  37. site directed mutagenesis requires restriction nucleases:
    a. true
    b. false
    b. false
  38. the glucose transporter:




    A. (a and c)
  39. the action potential in neurons is generated by:



    B. (Na+ channels)
  40. the Na+/K+ pump:




    D. (a and b)
  41. animal cells expend approximately _______% of their total ATP to transport Na+:



    C. 30%
  42. coupled membrane transport:



    C. (a and b)
  43. the active glucose transporter:




    D. pumps glucose against the concnetration gradient
  44. In intestinal epithelial cells:


    B. glucose is pumped in by active transport and out by passive transport
  45. plant cells modulate their ionic balance:



    C. by pumping H+ out of the cell
  46. auditory hair cell sterocilia transform sound waves into an electrical signal with this ion channel:



    D. Ca++
  47. the action potential in neurons has a maximum amplitude of about:



    B. +40 mV
  48. the toxin ouabain:



    B. inhibits K+ binding to the Na+/K+ pump
  49. neurotransmitters include:




    E. all of the above
  50. the toxin curare:



    C. causes muscle paralysis
Author
iishvo
ID
251385
Card Set
cell bio exam III key
Description
cell bio davis exam III
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