cell bio exam III key

  1. the following DNA nuclease produces straight, blunt ended cuts:

    C. HaeIII
  2. dissociation of dsDNA into single strands:

    A. (b or c)
  3. DNA from different individuals varies in:

    B. (a and c)
  4. leaky mitochondrial membranes:

    C. result in cell death
  5. cell membranes from the following organelles are exposed to the external environment:

    D. none of the above
  6. phosphatidylcholine contains:

    A. a quaternary ammonium ion
  7. phosphatidylserine contains:

    C. terminal amino acid and b. an ammonium ion
  8. K+ ion concentrations:

    A. are 30 times higher inside the cell than outside
  9. the passive transporter for glucose:

    A. works with the concentration gradient
  10. the maximum resting membrane potential for an animal cell is approximately:

    A. -0.2 V
  11. the following dye is used to visualize DNA bands after separation on agarose gels.

    A. ethidium bromide
  12. the polymerase chain reaction requires:

    D. all of the above
  13. after 3 cycles of PCR, the number of dsDNA strands resulting from one initial dsDNA strand is:

    B. 8
  14. reagents necessary to produce a recombinant DNA construct for use in E. coli include:

    E. all of the above
  15. from what is cDNA made?

    C. mRNA
  16. the restriction nuclease HindIII produces:
    a. straight blunt ended cuts
    b. staggered cuts with "sticky ends"
    b. staggered cuts with "sticky" ends
  17. DNA can be quantitated by measuring absorbance at _______nm:

    C. 260
  18. Taq polymerase is obtained from the organism:

    A. thermus aquaticus and c. bacillus thermoaquaticus
  19. eucaryotic systems for recombinant gene expression include:

    B. (a and b)
  20. patterns of gene expression in different cell types can be determined by:

    C. replacing genes of interest with reporter genes
  21. genetic manipulations include:

    D. all of the above
  22. a convenient source of natural membranes for experimentation is:

    B. red blood cells
  23. membrane scaffolding proteins include:

    E. (b and c)
  24. integral membrane proteins can be extracted with:

    D. (a and c)
  25. bacterial rhodopsin uses _________ to absorb light energy.

    C. retinal
  26. cells can complex glycolipids:

    A. as recognition motifs
  27. Concanavalin A is a(n):

    C. lectin
  28. Membrane channels are selectively permeable due to:

    B. (a and b)
  29. Ions with hydration shells can be transported by channels:
    a. true
    b. false
    b. false
  30. voltage gated Ca2+ channels:

    B. stimulate neurotransmitter release
  31. membrane channel control mechanisms include:

    B. (a and b)
  32. The nernst equation for membrane potenial is:

    A. V = 62 X log10(co/ci)
  33. the resting membrane potential is dependent primarily on _______ on both sides of the membrane. 

    C. [K+]
  34. the primary advantage of cDNA is:

    B. lack of introns
  35. restriction mapping of genomal segments:

    C. (b and c)
  36. Microarrays:

    D. (b and d)
  37. site directed mutagenesis requires restriction nucleases:
    a. true
    b. false
    b. false
  38. the glucose transporter:

    A. (a and c)
  39. the action potential in neurons is generated by:

    B. (Na+ channels)
  40. the Na+/K+ pump:

    D. (a and b)
  41. animal cells expend approximately _______% of their total ATP to transport Na+:

    C. 30%
  42. coupled membrane transport:

    C. (a and b)
  43. the active glucose transporter:

    D. pumps glucose against the concnetration gradient
  44. In intestinal epithelial cells:

    B. glucose is pumped in by active transport and out by passive transport
  45. plant cells modulate their ionic balance:

    C. by pumping H+ out of the cell
  46. auditory hair cell sterocilia transform sound waves into an electrical signal with this ion channel:

    D. Ca++
  47. the action potential in neurons has a maximum amplitude of about:

    B. +40 mV
  48. the toxin ouabain:

    B. inhibits K+ binding to the Na+/K+ pump
  49. neurotransmitters include:

    E. all of the above
  50. the toxin curare:

    C. causes muscle paralysis
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cell bio exam III key
cell bio davis exam III