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victimsofadown
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Describe each enzyme w/ group and important information presented?
- Amylase: Jodi and co (linking arms group)
- specific and general acid/base
- Citrate synthase:
- general acid/base
- galactokinase:
- glutamine synthase: Unicorn group
- Glutamate is P'd, but is ultimately removed to form Glutamine
- hexosaminidase A (hex a):
- General and specific acid/base
- Urease: The Dusos (muggers)
- Metal catalyzed, and general Acid/base
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What is the mechanism for the enzyme that your group presented. Give a detailed answer, including important chemical structures.
- Enzyme binds ATP and galactose
- Asp186 (-) removes proton from OH on C-1
- Newly nucleophilic O- on C-1 attacks Pi
- ADP and G-1-P released
- Asp186 releases H due to pKa
*general base mechanism
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Give an example of an enzyme mechanism that has metal ion catalysis.
Urease
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Give an example of an enzyme mechanism that has general acid catalysis.
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5. Give an example of an enzyme mechanism that has general base catalysis.
- Citrate Synthase
- Galactokinase
- ?Glutamine synthetase
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Give an example of an enzyme mechanism that has covalent catalysis
None/chymotrypsin only
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Give an example of an enzyme mechanism that has specific acid or specific base catalysis.
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Which enzyme catalyzes a reaction that cleaves ATP, but phosphate is not on the final product?
Glutamine synthetase
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Which enzyme catalyzes a condensation of a two carbon molecule and a four carbon molecule?
Citrate synthase
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Which enzyme catalyzes a reaction that raises the pH for enhanced bacterial growth?
Urease
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Which enzyme catalyzes a reaction removes a sugar from a ganglioside
Hexosaminidase A
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Which enzyme cleaves an α(1-4) glycosidic linkage
Amylase
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Which enzyme catalyzes a reaction that cleaves ATP, and phosphate is on the final product?
Galactokinase
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