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lab quiz 4
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what is the function of EMB agar?
to identify fermentation of various sugars
what are the two sugars used in EMB agar?
lactose and sucrose
what does fermentation of sugars do in an EMB agar?
1-sugars encourage the growth of enteric bacteria
2-it differentiates them based on color reaction created when combine the dyes
what type of coliform does lactose support in EMB agar?
supports the growth of Escherichia Coli (E.coli)
what type of coliforms does sucrose support in EMB agar?
pathogens such as Proteus or Salmonella
what is the purpose of the Eosin and methylene blue dyes in EMB agar?
1-Inhibit the the growth of of gram positive organisms
2-react vigorously with lactose fermenters and turn the growth dark purple or black
the green metallic sheen in EMB is typical of what?
the presence of E.Coli
what colors do less aggressive lactose fermenters produce in EMB?
pink to dark purple
what is EMB agar selective for?
encourages growth of Gram(-) and inhibits G(+)
What is the purpose of phenol red broth?
to demonstrate acid and gas production of organism
what causes gas production in PR broth?
fermentation of carbohydrate in the tube
what does yellow broth, bubble in tube mean in Phenol Red Broth?
fermentation and acid production A/G
what does yellow broth no bubble in Phenol red broth mean?
fermentation but no gas A/-
what does red broth, no bubble in tube mean in the phenol red broth test?
no fermentation, no gas production -/-
what does pink broth, no bubble mean in the phenol red broth?
degradation of peptone;alkaline end products
what causes phenol red broth to turn pink?
deamination of peptone amino acids producing Ammonia
what is Simmons Citrate agar used for?
to see if organism can survive on sole carbon and nitrogen source
In the simmons citrate agar what is used for sole carbon source?
sodium citrate
in the simmons citrate agar what is used as the sole source of nitrogen?
ammonium phosphate
what color is bromthymol blue dye at 6.9ph?
green
what color is the bronthymol dye at 7.6ph?
blue
what does blue in citrate test mean?
citrate is utilized
what does no color change but organism growth on the citrate test mean?
citrate is utilized
what does no color change (stays green) and no growth of organism mean?
citrate is not utilized
what is the SIM test used for?
to determine
1-sulfur reduction
2-indole production from tryptophan
3-motility
what does doe the color black in the SIM media mean?
sulfur reduction and a positive test
what does a layer of red mean in the SIM test?
it indicates positive for indole and tryptophanase presense
what effect does UV light C have on bacteria?
its detrimental, expose to UV-C causes irreparable DNA damage to organism
Leukocytes are two divided into which two groups?
1-granulocytes
2-agranulocytes
what are the different types of granulocytes?
1-neutrophils
2-basophils
3-eosinophils
what is a typical trait in a neurophil?
segmented nucleus
what percent of neutrophils make up WBC?
55-65%
what is a typical trait of basophil?
dark purple cytoplasmic granules that obscure the nucleus
what percent of basophils make up the total number of WBC?
.05-1%
what is a typical trait of eosinophils?
2x as big as RBC and contain two lobes that tends to make them look like aliens
what percent of Eosinophils make up the total number of WBC?
1-3%
how many types of agranulocytes are there?
two
1-monocytes
2-lymphocytes
what is a typical trait of a monocyte?
a round large indented nucleus
what percent of monocytes make up the total number of WBC
3-7%
what is a typical trait of a lymphocyte?
large nuclues with a thin halo of cytoplasm encircling their nucleus
what is the percent of lymphocyte that make up the total number of WBC?
25-33%
what are the WBC cells of the immune system?
lymphocytes
what are the functional types of lymphocytes?
1-T-cells
2-B-cells
3-larger natural killer cells
what group and type of cell is this?
granulocyte and its a basophil
what group and type of cell is this?
Granulocyte and its a eosinophil
what group and type of cell is this?
Agranuloyte and its a lymphocyte
what group and type of cell is this?
Agranulocyte and its a monnocyte
what group and type of cell is this?
granulocyte and its a neutrophil
Author
nando54321
ID
250065
Card Set
lab quiz 4
Description
Weeks 8-10
Updated
2013-12-02T02:36:17Z
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