Microbiology Lab Practical

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  1. what does the term ubiquitous mean?
    It means the organism can be found just about everywhere
  2. what does morphology mean?
    the study of the form and structure of organisms
  3. what is the purpose of flaming the tube?
    to sterilize the tubes tips and surrounding air
  4. where does the sterilization of inoculating instruments take place?
    in the tip of the inner flame
  5. what is the purpose of streaking a plate?
    to isolate a an individual cell from a mixed culture into a single cell that can form CFU
  6. how many quadrants does streak plate methods utilize?
    four quadrants
  7. what types of ribosomes so prokaryotes have?
    70s (30s and 50s sub units)
  8. what is the typical size of a prokaryote?
  9. what size Ribosomes do Eukaryotas have?
    80s (40s and 60s sub units)
  10. what is the DNA of prokaryote look like
    a single, circular molecule called chromosome
  11. what is the typical size of a eukaryote?
  12. what type of DNA do prokaryotes have?
    two to many linear molecules, each being a chromosome
  13. what are the name and shapes of the organisms?
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  14. what is the shape and arrangement of this organism?
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  15. what does purple color on cell wall mean?
    it is gram positive
  16. what does red-ish color of cell wall mean?
    that it is gram negative
  17. what is saboraud agar selective for?
    it is selective for Fungi
  18. what it MS Agar?
    an agar that contains Mannitol and salt and red phenol red
  19. what is the purpose of the mannitol in the agar?
    to reveal the presence of Sa, who is capable of fermenting the mannitol, which produces acid which turns the phenol red into a yellowish color
  20. what is the purpose of the salt in the agar?
    the salt is selective for staphylococcus which tolerates the salty environment of our skin, it also inhibits the growth and kills other bacteria not capable to surviving in an alkaline environment
  21. what is the purpose of using a blood agar?
    do determine the different types of alpha, beta and gamma hemolysin
  22. What is an EMB agar?
    an agar that contains Eosin and methylene blue
  23. what is EMB used for?
    inhibit the growth of Gram negative cells and to show reaction of lactose fermenters
  24. what is EMB selective for?
    Gram negative cells
  25. what is EMB differential for?
    Lactose fermentation
  26. what does a positive result of fermentation show?
    metallic green would show vigorous fermentation
  27. what organism would show a positive test for EMB?
    E. Coli and salmonella
  28. What is the purpose of Bile Esculin
    bile cleavage allows reaction with Fe to take place
  29. what does Bile Esculin distinguish for?
    group D strep
  30. what is Bile Esculin selective for?
    enterococci/group D strep
  31. what does a positive test for Bile Esculin look like?
    darkening of plate
  32. what does PEA agar plate stand for?
    Phenyl Ethyline Alcohol
  33. what is the purpose of using a PEA plate?
    It is used to break down the outer membrane of gram negative cells making the place selective for growth of Gram Positive cells
  34. what is the purpose of using a coagulate test?
    to find the presence of Sa which contains chemicals that causes blood to coagulate
  35. what does being positive for coagulate test means?
    it means that Sa is present
  36. what does positive for coagulate look like?
    a thicker, jelly like liquid
  37. what is the purpose of using Thioglycollate Media?
    it is used to sort out the type of aerotolarent organisms
  38. what type of bacteria grew in this environment?
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    obligate aerobe bacteria
  39. what type of bacteria grew in this environment?
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    obligate anaerobe
  40. what type of bacteria grew in this environment?
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  41. what type of bacteria grew in this environment?
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  42. what type of bacteria grew in this environment?
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    facultitive anaerobe
  43. how does the gaspak work?
    it contains no oxygen, allowing only strict anaerobes to grow
  44. how does the candle jar work?
    it burns off oxygen and produces high CO2, an ideal growth environment for microaerophiles
  45. what is the snyder agar used for?
    used to show amount of oral bacteria
  46. how does the snyder agar work?
    it allows bacteria to ferment or feed on sucrose which produces acid, causing the agar to green to yellow, the more yellow the more bacteria is present
  47. what is the catalase test used for?
    it is used to see if bacteria contains enzyme catalase and aerobic respiration
  48. what does the catalase distinguish between?
    staph which is gram positive and step which is gram negative
  49. what does a bubble in the inner closed tube mean in a catalase test?
    it means the enzyme catalase reacted with the hydrogen peroxide, causing a bubble to show in test
  50. how can you tell if the catalase test is positive for aerobic respiration?
    by the bubble in the tube
  51. what is the purpose of phenol red broth?
    to verify if organism can produce acid which will turn the broth from red to yellow
  52. what does the production of acid do to the ph balance?
    it decreases the Ph
  53. what does SIM stand for?
    Sulfur Indole Motility test
  54. what does SIM test for?
    • 1-it test for enzymes the reduce sulfur to H2S
    • 2-the indole test for the presence of tryptophan
    • 3-needle inoculation test for motility
  55. what does the Durham tube test for?
    it test for gas production
  56. what is citrate sodium medium used for?
    it is used to see if organism can utilize sodium citrate as the only source of carbon
  57. what is the color of a positive result on the citrate test?
    bright blue
  58. what color is a negative result for citrate test?
  59. what type of gram wall does the API 20E test system test on?
    gram negative only
  60. what is the Kirby-bauer test?
    its a test that utilizes many antibiotic disc on one agar plate
  61. what does the Kirby-Bauer disc test for?
    it test for antibiotic resistance
  62. what is an indication of antibiotic resistance on the petri dish?
    a clear hallow around the disc
  63. what color is the capsule stain?
    a white hallow around the capsule
  64. what color is the spore stain?
    gram positive with green circles
  65. what does vegetative state of bacteria mean?
    it means that its in its live stage
  66. what does magnification do on a microscope?
    it makes something appear larger
  67. what does resolution on a microscope do?
    it give clarity
  68. define sterile
    nothing living
  69. define contaminated
    something is the sample that you don't want
  70. what color is the basic stain?
    pinkish with white background
  71. what is a negative stain?
    when negative charge stain is added to bacteria causing the capsule to repel the stain and producing and white hallow around the capsule
  72. what color is gram positive stain?
    purple if positive and pinkish if negative
  73. what is an acid fast stain?
    a stain that checks for mycolic acid in the cell wall
  74. what does mycolic acid in cell wall do?
    it resists decolorization so its able to hold color stain despite being washed with acid alcohol
  75. what type of gram cells usually contain mycolic acid?
    gram positive cells
  76. what color is a positive result for mycolic acid?
    pink in the cell wall
Card Set
Microbiology Lab Practical
Lab practical m
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