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Where are all AA derived from?
Intermediates in glycolysis, TCA cycle or pentose phosphate pathway
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How does nitrogen enter Glycolysis, TCA, and Pentose shunt?
Via glutamine and glutamate
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How many AA can Bacteria/Plants/Animals synthesize
- Plants/bacteria: All 20
- Animals: Can only synthesize 10 simple AA, need to eat the rest (the ones eaten are the essential aa)
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Overview of AA Biosynthesis
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General Transamination Reaction
- Carried out by aminotransferase
- Switches O and NH3 f carboxylic acid and amine
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Role of alpha-keto glutarate
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Non-essential AA pathways
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3-Phosphoglycerate Family (Ser, Gly, Cys)
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Arginine Synthesis
- Comes from alpha-ketoglutarate
- Via Urea cycle
- Children need it, adults make enough
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Synthesis of Tyr, Phe, Trp
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All amino acids derived from what intermediates:
Glucose, 3-Phosphoglyceric Acid, Phosphoenolpyruvic acid, Pyruvate, alpha-KG, OAA
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Essential Human Amino Acids
- Val, Met, His, Leu, Phe, Lys, Ile, Thr, Trp, Arg
- Very Many Hairy Little Pigs Live In The Torrid Argentina
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Porphyrin Biosynthesis
- Glycine is basic part
- Aminolevulinate is used in repetitive condensation reactions to make porphyrin
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Heme B and Chlorophyll
- Porphyrins:
- Heme b has cofactor Iron, Chlorophyll has cofactor Mg
- Chlorophyll also has fatty acid chain to help anchor it
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