What is the 2 component of proton-motive force?
Chemical potential energy and Electrical potential energy
Chemical Potential Energy
Due to the difference in concentration of a chemical species (H+) separated by a membrane
Electrical Potential Energy
Results from a separation of charge across a membrane
2 basic postulates of Chemiosmotic model
1. electrons flow through transport chain, Energy released create proton gradient across inner mitochon membrane
2. protons move back across inner mitochon membrane release E that drive syntheses of ATP.
Venturicidin or oligomycin
Inhibitors of ATP synthase
Uncoupler, membrane soluble weak acid.
Can be synthesis in the absence of an oxidizable substrate, proton-motive force is sufficient to drive ATP synthesis
Complex V. proton-translocating multisubunit, transmembrane protein.
What are the 2 functional domain of ATP synthase?
: integral to membrane
: peripheral to the membrane
ATP synthase F1 complex
Each of the 3 beta subunit has one catalytic site for the ATP
ATP synthase F0
Make up the proton pore and is composed of subunit ab2c10-12
What are the 2 specific systems that transport ADP and P into the mitochon matrix and ATP out to cytosol
1. Adenine nucleotide translocase
2. Phosphate translocase
Primarily used by heart, kidney and liver. 2.5 ATP generated by pair of electrons that are passed from NADH to O2
Glycerol 3-Phosphate shuttle
Used by skeletal muscle and brain.
How many ATP does G-3-P shuttle provide
1.5 ATP molecule per pairs of electrons.