biochem-lec29.txt

Chemical potential energy and Electrical potential energy

Due to the difference in concentration of a chemical species (H+) separated by a membrane

Results from a separation of charge across a membrane

• 1. electrons flow through transport chain, Energy released create proton gradient across inner mitochon membrane
• 2. protons move back across inner mitochon membrane release E that drive syntheses of ATP.

Inhibitors of ATP synthase

Uncoupler, membrane soluble weak acid.

Can be synthesis in the absence of an oxidizable substrate, proton-motive force is sufficient to drive ATP synthesis

Complex V. proton-translocating multisubunit, transmembrane protein.

• F0: integral to membrane
• F1: peripheral to the membrane

Each of the 3 beta subunit has one catalytic site for the ATP

Make up the proton pore and is composed of subunit ab2c10-12

• 1. Adenine nucleotide translocase
• 2. Phosphate translocase

Primarily used by heart, kidney and liver. 2.5 ATP generated by pair of electrons that are passed from NADH to O2

Used by skeletal muscle and brain.

1.5 ATP molecule per pairs of electrons.