microbiology lab day 8 part 1 (kirby-bauer test, MIC, microbiostatic, MBS, antibiotics, phenol coeff

antibiotics diffuse slowly from the disks to the agar plates

antibiotics

the bacterium is senstitive to this antibiotic

Bacterium is plated using a spread-plate technique and then antibiotic disks are stamped onto the plate. Then the plate is innoculated and examined for zones of inhibition

minimum inhibitory concentration. The minimal concentration of an antibiotic that inhibits the growth of a bacterium

an inhibitory chemical

either no zone of inhibition or a very small zone of inhibition

the ability to deter a bacterium's growth by using a particular MBS agent

It is determined with regard to the phenol coefficient

(highest dilution (i.e. lowest concentration) of antimicrobial of interest)/(highest dilution of phenol with same microbicidal results)

anaerobic pathogens

a petri dish containing the proper agar is streaked in three different directions with the test bacterium and special plastic Etest strips are placed onto the surface so that they extend out radially from the center. Each strip represents a different antibiotic and it contains a gradient of the antibiotic