microbiology lab days 5 and 6 part 1 (biochemical assay, fermentation, beta galactosidase, onpg, TSI

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what is fermentation?

an energy producing biochemical reaction i nwhich organic molecules serve as both electron acceptors and electron donors.
Phenol
red broth is used to detect ________ in bacteria

fermentation
Phenol red is _____ below pH 6.8,_____ above
7.4, and ____ between these two points.

yellow, purple-pink, and red
a ____ ___ is used to detect whether or not a bacterium produces gas

durham tube
how do you tell what the results are for a fermentation test?

red color no gas - no fermentation

yellow color no gas - acid production

yelow color and gas - acid and gas production

red color and gas - alcohol fermentation
what does B-galactosidase do?

it breaks down lactose to galactose and glucose
what chemical is used in B-galactosidase detection?

ONPG
how does the B-galactosidase test work?

ONPG is cleaved into o-nitrophenol by B-galac., which yields a yellow color.
what is a key tablet used for?

it can be used to detect B-galc. activity. If it turns yellow B-galac. is present
TSI is used to identify and distinguish the _____ from other ___ ___ organisms

enterobacteriaceae from other gram negative organisms
What is contained in the agar in the Triple Sugar Iron Agar test?

1% lactose and sucrose, 0.1% glucose, Phenol red
acetoin is a precursor of

2,3-butanediol
barritt's reagent is used in what test?

voges proskauer
What type of agar is used in gelatin hydrolysis test?

nutrient gelatin deep tubes
what is a positive gelatin test?

liquified medium

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mikepl103

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microbiology lab days 5 and 6 part 1 (biochemical assay, fermentation, beta galactosidase, onpg, TSI

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microbiology lab days 5 and 6 part 1 (biochemical assay, fermentation, beta galactosidase, onpg, TSI, imvic, gelatin, catalase, coagulase, dnase, oxidase)

Updated

2013-11-03T23:10:22Z

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