microbiology lab day 7 part 2 (day 7 bacterial growth curve, conjugation, Lederburg, Tatum, anaerobi

Brewer's special cover fits on a normal petri plate bottom in such a way that its circular ridge rests on the agar, thereby protecting most of the surface from exposure to O2.

it contains a high concentration of thioglycollic acid.

the free sulfhydryl groups of the thioglycollate reduce any O2 present and create an anaerobic environment under the Brewer cover.

hydrogen gas and co2 are generated by a gaspak envelope after the addition of water.

it catalyzes the formation of water from h2 and o2, thereby removing o2 from the sealed chamber.

An envelope is added to a chamber. hydrogen and co2 are generated by a gaspak envelope after the addition of water. Once water is added to the envelope and it is put into the chamber, a lid is put over the chamber. Inside of the chamber is also a palladium catalyst which catalyzes the formation of H2O by combining H2 and O2.

living bacteria

25 and 250

it is a way of analyzing the amount of bacteria in a medium. Light is passed through the medium and if there is a high population in the medium, then this means that less light is transmitted through. The light that is transmitted through is then converted to electrical energy and is measured by a galvonometer.

Lederburg and Tatum

• the transfer of DNA by direct cell-to-cell
• contact between bacterial cells

horizontal gene transfer

conjugative or mobile genetic elements

• a conjugative plasmid that plays a major role in 
• E coli