TOB Integument (12)

  1. functions of the integument (skin)
    • 1. protective, waterproof barrier (keratin, hair, sebum)
    • 2. maintains body temperature through capillary dilation/contraction & production of sweat (thermoregulation)
    • 3. major sensory input for pain, pressure, heat & cold
    • 4. performs important metabolic functions (eg. site of vitamin D synthesis)
    • 5. immunological (contains langerhan's cells [DC], wandering lymphocytes)
    • (6. absorption: nicotine, steroid hormones)
  2. the skin consists of two layers:
    • 1. epidermis: SSKE (stratified squamous, keratinizing epithelium)
    • 2. dermis
  3. epidermis
    • outer layer of the integument firmly attached to the underlying dermis
    • made up of distinct layers
    • this region is enriched with nerve endings, is AVASCULAR & has a stratified squamous keratinizing epithelium
    • epidermis keratinocyte layers exterior --> inward (apical --> basal)
    • 1. Stratum Corneum
    • 2. Stratum Lucidum
    • 3. Stratum Granulosum
    • 4. Stratum Spinosum
    • 5. Stratum Basale (Germinativum)
    • (Come Let's Get Sun Burned)

    Image Upload 1

    Image Upload 2
  4. Stratum Corneum [come]
    • outermost region contains many layers of flattened, cornified, dead cells whose cytoplasms are packed with TONOFIBRILS
    • number of cell layers varies with the thickness of the skin (more layers in thick skin like palms & soles of the feet)
  5. Stratum Lucidum [let's]
    thin translucent layer apparent in some sections of thick skin, but not always present (possibly an artifact)
  6. Stratum Granulosum [get]
    • contains 2-3 layers of flattened squamous epithelial cells whose cytoplasms are filled with coarse basophilic (keratohyalin) granules
    • granules contain filaggrin (binds tonofilaments into tonofibrils) and loracrin (thickens/stiffens the outer surface of cells)
    • last layer before the cells "die"
  7. Stratum Spinosum [sun]
    • THICKEST of the living layers of the epidermis
    • cells connected by abundant desomosomes
    • have a spiny appearance
    • Image Upload 3
    • Image Upload 4
  8. Stratum Basale (Germinativum) [burned]
    • innermost layer of cells rest on basement membrane & are single layer of cuboidal cells
    • these cells divide regularly meaning a high number of mitotic figures are visible
    • they move upward & replenish cells lost at the surface during epidermal turnover
  9. keratinocytes
    cells of the epidermis that contain abundant water insoluble keratin assembled into 7-8 nm tonofilaments inside their cytoplasm
  10. dermal papilla
    Image Upload 5
    • finger like projections of the dermis into the epidermis which enhance the attachment of the epidermis to the underlying dermis
    • these structures generate an enormous surface area for strong epidermal-dermal interaction
    • stem cells are located near the tips of the dermal papilla

    • Image Upload 6
    • primary ridge: encompassing single ridge over two¬†dermal papilla
  11. dermis
    connective tissue layer that lies deep to the epidermis and consists of a highly vascularized connective tissue with different types of glands, hairs and sensory structures
  12. hypodermis (superficial fascia)
    • lies deep to the dermis, but is not technically a part of the skin
    • several structures associated with the true skin (eg. hair follicles, sensory receptors) may be found in this region
    • hypodermis contains adipose tissue & may contain skeletal muscle
  13. cells of the epidermis
    • 1. Keratinocytes
    • 2. Melanocytes
    • 3. Langerhan's Cells
    • 4. Merkel Cells
  14. Melanocytes
    • pigment producing cells derived from the neural crest found in the basal layer
    • have long processes containing melanosomes that extend among the keratinocytes
    • melanosomes = membrane-bound organelles that make pigments (melanin and phaeomelanin)

    Image Upload 7
  15. Do keratinocytes synthesize pigment?
    • NO, melanocytes do
    • keratinocytes phagocytose the pigment-containing cytoplasm of the melanocyte and ACQUIRE pigment
  16. What might increase melanocyte activity?
    exposure to UV light as well as by melanocyte stimulating hormone (MSH)
  17. Langerhans Cells
    • dendritic cells, functionally related to the monocyte-macrophage group (and thus derived from the bone marrow) found particularly in the upper layers of the stratum spinosum
    • present antigen to lymphocytes
  18. Merkel Cells
    • cells involved in sensory (mechano) reception & contain granules similar to those found in the cells of adrenal medulla
    • occur in small numbers in the basal part of the epidermis
  19. psoriasis
    • caused by pathological changes that lead to over-proliferation of the stratum basale layer
    • can lead to basal cell carcinoma (most common form of skin cancer) if the changes are neoplastic
  20. melanoma
    • a much more aggressive form of skin cancer caused by neoplastic changes in the melanocytes
    • responsible for the majority of skin cancer deaths
    • Initial diagnoses are usually made based upon visual appearance w/ mnemonic ABCDE:
    • A - asymmetry
    • B - Border (irregular)
    • C - Color (usually multiple)
    • D - diameter (> 6mm)
    • E - enlarging
    • good prognosis w/ excision of early identified tumors
  21. What are the two laybers of the dermis
    • 1. papillary
    • 2. reticular
  22. Papillary Dermis
    • layer just deep to the epidermis composed of finger-like papilla that project toward the surface, forcing epidermis to acquire an undulating morphology
    • this greatly increases the surface area for epidermal-dermal adhesion
  23. Why do papilla contain sensory nerve endings & capillary beds?
    because they bring the dermis as close to the outside world as possible, an ideal location for two such entities
  24. Reticular Dermis
    • constitutes the bulk of the dermis & contains large bundles of type I collagen fibrils [Langer lines] + elastic fibers
    • reticular dermis contains sensory receptors, hairs & several types of glands
  25. What are 3 glands of the skin?
    • 1. Eccrine Sweat Glands
    • 2. Apocrine Sweat Glands
    • 3. Sebaceous Glands

    Image Upload 8
  26. Eccrine SWEAT Glands (merocrine)
    Image Upload 9
    • a basal coil from which a duct leads through the dermis into the epidermis assists in maintaining a normal body temperature
    • secretory portions of eccrine sweat glands pump Na+ ions into the lumen to generate an osmotic gradient; water follows by osmosis. In addition to water, several protein components (e.g. dermcidin) and other waste products (urea) are added to the fluid
    • ductal cells actively resorb sodium & passively reabsorb chloride; the final product to reach the surface is mostly water & a small amount of protein/sodium
    • are found over most of the body but most concentrated in the forehead, axilla & palms of the hand

    Image Upload 10
  27. What are eccrine sweat gland ducts made of?
    stratified cuboidal epithelial cells rich in mitochondria
  28. cystic fibrosis
    • patients have compromised removal of salts by eccrine sweat gland ducts
    • --> a high salt content in sweat = marker of CF
  29. Apocrine Sweat Glands
    Image Upload 11
    • large coiled tubular glands that open into hair follicles rather than directly onto the skin surface
    • found mainly in the axilla (armpit) & genitals
    • less numerous than eccrine glands
    • apocrine glands can store their secretion, & various hormonal/neural stimuli will cause them to secrete
  30. What is the likely primary functions of apocrine glands?
    • to produce pheromones
    • they become active at puberty
    • mode of secretion = eccrine and apocrine (the secretory product as well as a portion of the cells themselves is released)
  31. Which is apocrine and which is eccrine?
    Image Upload 12
    • left: apocrine - larger lumen for sweat storage
    • right: eccrine - smaller lumen, no storage
  32. Sebaceous Glands
    • glands found all over the body EXCEPT at palms & soles that secrete sebum, which serves a natural lubricant of the hair and surface of the skin (contains TAGs, FAs, & cholesterol)
    • sebaceous glands discharge contents via a SINGLE duct into the lumen of hair follicles
    • mode of secretion = holocrine (entire cell & its product is released)
  33. Where do sebaceous glands secrete sebum directly onto the skin surface?
    • fordyce spots (penis bumps)
    • lip vermillion border
  34. Acne
    • caused when sebum production (puberty) exceeds the capacity of the short ducts to drain the glands
    • can become colonized by surface bacteria
    • while bacterium is a normal component found in healthy skin, prolonged colonization of sebaceous glands results in the release of lytic enzymes and damages gland & ducts
  35. Hairs
    dead epithelial fibers composed of cross-linked keratin that is harder than that found on the skin, owing in part to the use of trichohyalin rather than filaggrin to bind tonofilaments into tonofibrils
  36. What are the three parts of hair?
    • 1. shaft: part projecting from the skin surface
    • 2. root: the portion embedded in the skin
    • 3. follicle: the tubular invagination of the epidermis
  37. What time of muscle do most hairs have associated with their follicles?
    • smooth muscle (arrector pili)
    • contraction causes the hairs to become erect (goose bumps)
    • arrector pili are absent in axilla, groin, eyelashes & eyebrows
  38. What are the 3 phases of hair growth?
    • 1. anagen (a growth phase that may last for years)
    • 2. catagen (a follicular regression stage which lasts weeks)
    • 3. telogen, (a resting period lasting a few months).
  39. What is responsible for the perception of touch, temperature, pressure and pain?
    • free nerve endings abundant in the epidermis
    • skin at every part of the body functions as a sensory receptor
  40. Meissners corpuscles
    Image Upload 13
    • rapidly adapting sensitive organs found in dermal papillae that confer high discrimination
    • they stop firing after continued stimulation --> why we soon become unaware of the clothes touching our skin
    • used for tasks such as reading braille
  41. Pacinian corpuscles
    Image Upload 14
    rapidly adapting sensory organs that respond to deep touch pressures (massage) found in the deep dermis and hypodermis
  42. Image Upload 15
  43. Image Upload 16
    • stratum spinosum
    • rail roads between cells --> desmosome connections between cells
  44. Image Upload 17
  45. Image Upload 18
    Langerhan's cells contain Birbeck granules (function unknown)
  46. Image Upload 19
    • eccrine gland
    • dark = ducts (stratified cuboidal)
    • light = gland
    • thin surrounding gland = myoepithelium
  47. Image Upload 20
    • clear bubble = sebaceous gland
    • thin pink line =¬†smooth muscle (arrector pili)

    all next to hair bulb/follicle

    Image Upload 21
Card Set
TOB Integument (12)
Exam 3