Lab quiz 3
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What are stains
solutions consist of solven and colored molecule,
What is a chromophore?
the portion of the chromogen that gives chromogen its color.
What is auxochrome?
of a chromogen
allows chromogen to act as a dye through ionic or covalent bonds between chromogen and the cell.
What are basic stains?
ochromes become positively charched.
ttracted to the negative charge
s on the surface of most bacterial cells
What does heat-fixing do?
kills the bacteria
and makes the
to the side and coagulates cytoplasmic proteins to make them more visible.
Distorts the cell to
What do Differential and structural (Gram stains) allow microbiologist to detect?
differences between parts of the same organism
deetermince cell size, morphology, and arraignment.
What are the steps to gram staining?
Primary stain (crystal violet)
Iodine added as
, enhance crystal violet (forms crystal violate-iodine complex.
gp not decolorized by acetone
gn decolorized by counterstain safranin.
Lab quiz 3
gram stain 3