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Luria, Bertani, Weigle
Obsevered some strains of e.coli were resistant to Lambda phage
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Werner Arber
- Identified Restriction due to cutting of lambda DNA
- Observed methylation
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Arber, Linn, Meselson and Yuan
Isolated EcoK (first restriction endonuclease)
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Hamilton Smith
Isolated H. Influenzae retricion enzyme (HindI)
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Daniel Nathans
Restriction map of SV40 with HindIII
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Sharp, Sugden, Sambrook
Something to do with Agarose gel
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Robert? Southern
Southern Blotting
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Kary Mullis
Spoke to a green glowing Raccon
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P. Berg
Ligated plasmid and phage DNA using added A's and T's
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S. Cohen, A.Chang
Used Mendel and Higa's technique to introduce antibiotic resistant plasmid to e. coli
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Boyer, Cohen and Chang
Used sticky ends produced by restriction enzyme to create recombinant plasmid
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Birnboim and Dolly
Alkaline Lysis
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