Vg1 paper exam 1.txt

  1. Vg1 role
    • Member of the TGF-B family
    • the ONLY TgF-B protein that is localized DORSALLY
    • Needed for ANTERIOR development!
    • Located in VEGETAL Cytoplasm, which is where formation of mesoderm occurs
  2. Paper results related to Vg1 role
    • Although Vg1 overexpression does not cause mesoderm formation, LOSS of Vg1 causes loss in mesoderm formation and DELAYED GASTRULATION
    • An allele made with Serine residue is able to rescue effects of Vg1 loss of function, better than inserting the original Vg1 transcript.
  3. Vg1 and signalling
    • 1. Vg1 signaling NEEDED for phosphorylation of SMAD2
    • 2. Vg1 signaling NEEDED to PREVENT Bmp signaling by phosphorylation of Smad1
  4. Vg1 results Fig 1
    • Showed that through stages of gastrulation, Vg1 depleted
    • Vg1 has NO affect on Xnr3 or siamois, which are required for Wny pathway activation, so Vg1 NOT needed for Wnt pathway
    • Mesoderm inducing signals chordin and cerebus REDUCED at mid-gastrula stages,
    • Mesoderm markers cerebus, chordin, noggin and signals FGF8 and Xbra are DOWNREGULATED with Vg1 Depletion
    • Vg1 depleted showed UPREGULATION of Bmp target gene Sizzled, but No change w BMP4
  5. Vg1 results Fig 2
    • changing amino acid Vg1(ser) showed HIGHER expression vs Proline
    • Serine able to rescue partially the Vg1 depleted affect, by increasing Smad2 protein back to WT levels
    • Vg1(S) rescued partially mesoderm markers cerebus, chordin, noggin and dkk
    • Vg1(S) rescued mesoderm signals FGF8, Xnr1, chordin and Xsox17a during gastrulation stages
    • Vg1(S) rescued Bmp target Sizzled, brought the levels back down (were up with depletion)
    • Vg1 seen at HIGHER proportion DORSALLY via Realtime and Western (when compared to ventral, with VegT as control for all over cell)
Author
rincrocci
ID
239438
Card Set
Vg1 paper exam 1.txt
Description
vg1
Updated