Histology of Bone (LAST SET, Part I) slides 116-140

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    •After collar bone is established•chondrocytes become hypertrophic•As chondrocytes enlarge •matrix is compressed•forming thin plates between chondrocytes•Next, the surrounding matrix calcifies•should not to be confused with bone calcification
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    A: Periosteum aka collar boneB: crowding and calcified cartilage, lack of nutrients/blood/O2C: causes cell death of chondrocytes in model-> matrix breaks down -> lacunae become confluent to produce large cavities.
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    blood vessels, accompanied by osteogenic cells, grow through thin collar bone to vascularize the cavities (periosteal bud)
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    Periosteum/ collar
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    Cartilage in the middle and osteogenic cells on outside- process of laying down bone.  See dark red area- calcified- lightly stained area is cartilage look at osteocytes in red area bone (pink is cartilage in middle- deposition of bone by osteoblast. Blood vessels are round structures.
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    A: Shaft of bone with spongy bone B: Collar bone with osteogenic cells making more bone
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    Section of endochondral ossification. (A) The osseous (bone) matrix, rich in collagen type I, is specifically stained with picrosirius-hematoxylin. The cartilaginous matrix (B), containing collagen type II, stains blue with hematoxylin because of its high content of chondroitin sulfate. C. is Artifact; ignore
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    • Endochondral ossification.  A: calcified cartilage.
    • B. megakaryocyte.   C: Bone
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    ENDOCHONDRAL OSSIFICATION B:Osteoclast on bone spicule.    C: spicule of calcified cartilage (light blue) and covering of D: bone tissue (dark blue).  B. Osteoclast has resorbed bone tissue and lies in B2. Howship’s lacuna. Light area between osteoclast and surface of bone is the (B3) ruffled edge of the osteoclast.  Arrows indicate osteoprogenitor cells on the non-growing surface.  On the opposite side, bone is deposited by osteoblasts with newly formed osteocytes just inside the bone matrix.
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    • A. Myeloid/ Osteoprogenitor/Osteogenic cells in medullary/marrow cavity
    • B. Osteoclasts
    • C. Osteblasts
    • D. Muscle cell
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    primary ossification center
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    • A: Bony Collar
    • B. Primary Ossification center
    • C: Epiphyseal plate
    • D: primary ossification center
    • E: Epiphyseal line
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    Mawhi's in-class drawing for review
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    • knee, PSH stain
    • A: Endochondral ossification
    • B. Epiphysis
    • C. Synovial membrane
    • D. Articular cartilage
    • E. Capsule
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    • A: Articular cartilage (bone is never made here)
    • B. Epiphysis
    • C. Epiphyseal plate cartilage (bone never made here)
    • D. Diaphysis
    • E. Primary ossification center/ marrow space
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    • A: articular surface (cartilage only)
    • B. Epiphyseal growth plate seen at either end of cavities (marrow)C. Secondary ossification center (marrow)
    • D. Primary ossification center/Marrow
    • D. Shaft/Spongy bone/ Growth cavity
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    Rapid bone remodeling.  OC, osteoclast; OB, oseoblast; OY, osteocyte.  OC- numerous at surface of bone., round globs with multiple nucleus attached to bone surface. HL- howship’s lacuna white zone OB is building OC is remodeling/resorbing
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    • 3 ARROWS: Osteoclasts attached to boneA: CENTRAL REGION: Red area: cytoplasmic processes called central region, ruffled border with hydroxyl apatite crystals
    • B: Clear zone (resorption): actin filaments form a zone at perimeter of cell at surface (ligher area) of osteoclast attachemnet to bone. Attached by focal adhesion.
Card Set
Histology of Bone (LAST SET, Part I) slides 116-140
Histology of Bone (LAST SET, Part I) slides 116-140