HISTO BLOCK II CARTILAGE

• 1. Capsular or pericellular matrix (arrow)
• -Highest conc of sulfated proteoglycans
• - Found in immediate vicinity of chondrocytes
• -Stains intensively with basic dyes

• 2- Territorial matrix (circled around the cells)
• -Surrounds the isogenous group  of chondrocytes
• -Stains less intensively
• -Lower conc of sulfate proteoglycans

• Interterritorial matrix (odd shaped circle)
• - Occupies the spaces between groups
• of chondrocytes.  Weak basophilia
• - Lowest conc of proteoglycans

Metachromasia showing capsular, territorial, and interterritorial maxtrix areas

ACTIVE chondrocytes show areas of basophilic cytoplasm (RER) and prominent Golgi (arrows)

Chondrocytes. Clear areas represent areas of glycogen and lipids

• Active chondrocyte
• A-rER
• B-Golgi
• C-Nucleus
• D-Mitochondria

Trachea is lined by a psuedostratified ciliated columnar epithelium (EP).  Chondrocytes (C) are visible within the mass of hyaline cartilage that make the tracheal ring.  The cartilage is covered from both sides by pericondrium (P).  V, blood vessel

• A-Perichondrium
• B-Chrondoblast

• A-perichondrium
• B-Chondrocyte
• C-chondroblast

• Hyaline cartilage
• A-pseudostratified ciliated columnar epithelium
• B-perichondrium
• C-chondrocytes

Perichondrium (arrows) surrounds a growing hyaline cartilage.

hyaline cartilage, surrounded by perichondrium

Hyaline cartilage is surrounded by perichondrium (Peri) which consists of two layers; outer fibrous layer (F) composed of mostly collagenous fibers and fibroblasts, and an inner more cellular chondrogenic layer (CG) that gives rise to chondroblasts (CB).  As newly differentiated chondroblast starts to secrete ECM they eventually became surrounded by their secretion and called condrocytes (CC).

hyaline cartilage incorporating fibers. silver stain

The surface of the articular cartilage (solid arrows) and the interface between the cartilage and bone (dashed arrows) are free of perichondrium.

elastic cartilage, resorcin stain

Elastic cartilage in middle of epiglottis

ELASTIC CARTILAGE. KEY: AT, adipose tissue; E, elastic fiber; EC, elastic cartilage; MG, mucous gland; P, perichondrium; SE, stratified squamous epithelium. Epiglottis human.  H&E plus orcein 1= X80;  2= X 250

Higher magnification of the elastic cartilage in the epiglottis.  P, perichondrium; CC, chondrocyte; F, elastic fibers.

elastic cartilage

epiglottis, elastic cartilage with perichondrium

Epiglottis.  Elastic cartilage.  Note the elastic fibers (F) among the condrocytes (C).  Adipocytes (A) appear at the left side (upper and lower corners).