Histology Connective Tissue Lecture 1 part I

  1. Image Upload 1
    • A:BV, blood vessel;
    • B: MeC, mesenchymal cell. Can see more than one nucleolus means it’s actively dividing.
    • C, cell cytoplasmic processes,  C is the elongation of cytoplasmic process. BV- blood vessel is highly vascularized in CT.
  2. Image Upload 2Where is this connective tissue sample from? What is the matrix called and made of? Is it Toti, Multi, Pluri or Uni potential?
    This is from umbilical cord. Matrix is called Wharton's Jelly and it is a collagen fibrous matrix full of pluripotential cells! (Stem cells)
  3. Image Upload 3Label
    Mucus connective tissue – umbilical cord. F, fibroblasts; CF, collagen fibers (eosinophilic brown bands).  The empty-looking spaces (arrowheads) are areas where the ground substance was extracted during specimen preparation. Cytoplasm is buried hidden by collagen fibers. Arrowhead space is the GROUND SUBSTANCE – macromolecules proteoglycans. Are washed out in preparation.
  4. Image Upload 4Is this loose or dense connective tissue?
    Mucous tissue of an embryo showing fibroblasts immersed in a very loose extracellular matrix composed of collagen fibers and molecules of the ground substances. H&E stain.
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    • A: Elastic Fibers
    • B: Collagen Fibers
    • Loose (areolar) connective tissue.  Whole mount preparation. WHOLE MOUNT PREPARATION not from plastic. Small glass slides, fixed, stained, NOT INFILTRATED with plastic. Here there are many types of cells but you can’t see. Elastic fibers are branching (not bundles). Underneath epithelium.
  6. Image Upload 6What are the arrows? What is dispersed in between the arrows?
    • Arrows: small blood vessels/capillaries
    • Section of loose connective tissue. Fibroblast nuclei are interspersed with irregularly distributed collagen fibers.
  7. Image Upload 7
    Is this loose or dense connective tissue? What is interspersed in this tissue sample? What kind of preparation? What is high lighted?
    LOOSE connective tissue. Blood vessel big and small. Muscle fiber. Loose (areolar ) connective tissue.  Whole mount preparation (no infiltration step with plastic). BRANCHING of elastic fibers highlighted.
  8. Image Upload 8Is this loose or dense CT? Where does it usually surround?
    Loose (aka areolar) connective tissue.   Mast cells (related to basilphils) for allergy response. Loose connective tissue facilitate diffusion of molecules and immunity response (macrophages, basilphils, neutrophils)
  9. Image Upload 9Label
    • A: Elastic Fibers (black, thin spindles)
    • B. Collagen fibers (brown thicker bundles)
    • This is loose connective tissue. Other cells interspersed, but you cannot see what they are.
  10. Image Upload 10Label
    • A: Elastic Fibers
    • B. Fibroblast (note elongated cells with nucleus)
    • C. Collagen fibers
    • D. Plasma cells (from B-Cell)
    • E. Endothelial cell (usually all these are "thrown" on top)
    • F. Mast Cell (from Basophil)
    • G. Macrophages (large with lysosomes)
    • H. Eosinophils (bi lobed, granulated)
    • I. Adipose Cell
    • J. Lymphocyte
  11. Image Upload 11
    • A. Fibroblast
    • B. Collagen Fibrils surround fibroblast
    • C. Fibroblast
    • D. Smooth muscle cell
    • E. Wall of blood vessel
    • F. Lumen of blood vessel
  12. Image Upload 12Label
    connective tissue matrix. The ground substance is a fine granular material that fills the spaces between the bandingpatterned rod- like collagen fibrils (C) and elastic fibers (E)and surrounds fibroblast cells (F)
  13. Image Upload 13
    Longitudinal section of regular dense connective tissue from a tendon. B: Thick bundles of parallel collagen fibers fill the intercellular spaces between fibroblasts. A: Higher magnification view of a tendon of ayoung animal. Note active fibroblasts with prominent Golgi regions (G), nucleus(N) and dark cytoplasm rich in RNA.
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    What kind of cell is this? Label. Where can you find cell nuclei?
    REGULAR DENSE CONNECTIVE TISSUE, TENDONS. KEY: BV, blood vessel; TC, tendon cell nuclei. dashed line, arbitrary cross-sectional cut of tendon. Nucleo visible as long lines.
  15. Image Upload 15What kind of connective tissue is this? What is BV?
    Dense regular connective tissue of tendon (cross section).  BV, blood vessel; Ent, endotendineum; TC, tendon cell nuclei.  Dark spots of TC.  Ent- don’t need to know but division of tenden.  Boxed cell magnified next slide
  16. Image Upload 16
    Electron micrograph of a fibrocyte (cross-sectioned) in regular dense connective tissue. The sparse cytoplasm of the fibrocytes is divided into numerous thin cytoplasmic processes that interdigitate among the collagen fibers. x25,000.
  17. Image Upload 17Label. What type of cell is this?
    • A: striated muscle
    • B. Tendon
  18. Image Upload 18
    Label. What cell type? What 3 places do you find this cell type?
    • A. Striated muscle cell
    • B. Fibroblast
    • C. Tendon
    • Regular Dense Connective Tissue
    • Tendons, ligaments, cornea
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    What type of connective tissue is this? Label arrow & arrowhead. Where can it be found?
    Section of immature dense irregular connective tissue. This figure shows numerous fibroblasts (arrow) with many thin cytoplasmic extensions (arrowheads). As these cells are pressed by collagen fibers, the appearance of their cytoplasm depends on the section orientation; when the section is parallel to the cell surface, parts of the cytoplasm are visible. Collagen fiber is random but still dense- allows EXPANSION and resist stretching/tearing. Found in intestine, scalp and other tissues that resist stretching & tearing.
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Histology Connective Tissue Lecture 1 part I