lysine iron agar

how is lysine iron agar differential

LIA is used to differentiate enterics based on their abilityto decarboxylate or deaminate lysine and produce hydro- gen sulfide (H 2 S).
the fermentable carbohydrate in lysine iron agar is

dextrose glucose
the primary substrate for deamination and decarboxylation reactions in this experiment is

lysine
the pH indicator in this medium is broncresol which is _____ at pH 6.8 and higher and___ at ph 5.2 and below
- 1.purple
- 2. yellow
thiosulfate when reduced becomes

reducible sulfer
ferric iron ion in this experiment reacts with hydrogen sulfide to for

black precipitate
this medium proves an ____ zone for growth on the slant and an ____ zone for growth in the butt
- 1. oxygen aerobic
- 2. no oxygen anaerobic
what are all typical results and interpretations in this experiment

Medium is darkened within 48 hours Presumptive identification as a member of Group D Streptococcus or Enterococcus1

No darkening of the medium after 48 hours Presumptive determination as not a member of Group D Streptococcus or Enterococcus
decarboxylation compare how he conditions oxygen levels are created with this experiment versus the moellers decarboxylase broth with lysine
what is the selective agent

; bile is the selective agent added to separate the Streptococcus bovis group and e nterococci from other streptococci.
Gram-negative enterics,

demonstrate tolerance to bile
fresh cultures

Lactococcus lactis Enterococcus faecalis surrogate (Serratia marcescens)

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justjay18xx

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lysine iron agar

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lysine iron agar

Updated

2013-09-18T07:51:22Z

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