bio 115

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what is the name of the microscope

Bright field light microscope
what does the bight field light microscope do

produces dark image against light background
who is the bright field light microscope most comminly used by

biology students
what is the bright field light microscope used for
- to view preserved stains
- which means dead specimen with dye on it
what is the framework of the microscope
- arm
- base
- head which rotates
what is the stage

fixed platform that supports the microscope slide
what is the mechanical stage
- allows horizontial and vertical movement
- allows to center the specimen
what is the light source
- its build into base
- round knob allows you to control intensity of light
what is the focusing knob

concentrically arranged knobs on side of microscope that brings specimen into focus
what are the focusing knobs on the microscope
- coarse adjustment
- fine adjustment
what is the coarse adjustment
- larger
- outer knob
- rough image
what is the fine adjustment
- smaller
- inner knob
- fine image
what are the 3 types of lenses
- ocular lens
- objective lens
- condenser
ocular lens
- eye piece
- binocular because it has 2
- magnification of 10x
objective lens

the 4 lens that are attached to rotating nosepiece that allows them to move over a slide
what are the 4 objective lens
- 4x
- 10x
- 40x
- 100x
4x
- scanning
- shortest
- red
10x
- low power
- yellow
40x
- high dry
- blue
100x
- oil immersion
- white
- need to use drop of oil
- lenses need to touch the oil for it to work
condenser
- below stage
- lens that collect and direct the light from the lamb to the slide being studied
what does the condenser do that the ocular and obective lens doesn't

does not effect the magnifying power
what is the part within the condenser and what does it do
- diaphragm which controls amount of light that reaches the slide
- also known as iris diaphragm
what are the 2 jobs of the microscope
- magnification
- resolution
what do we mean when we say one of the microscopes job is magnification

ability to enlarge objects
what do we mean when we say one of the microscopes job is resolution

capacity to distinguish or separate two adjacent objects as separate
what are the two phases that magnification occurs in

the objestive lens magnifies and then the ocular magnifies again to form an image
what is the formula to calculate total magnification

power of objective X power of ocular= total magnification
what is the purpose of oil
- to keep the light into image
- prevents refraction of light
what is the total magnification of each objective lens
- 4x=40x
- 10x=100x
- 40x=400x
- 100x=1000x
what are the steps to use the microscope
- center specimen
- start with low power objective (10x)
- turn coarse adjustment knob until image appears
- bring object into focus with fine adustment
- once image is visible progess to next objective
what do you never want to do after focusing the image with fine adjustment

do not readjust coarse objective knob
what sould you do before moving objective to oil immersion
- place a drop of oil on the slide
- go half way between objectives, put the drop if oil, then finish sliding objective into place
- should be touching oil
transport
- always carry with 2 hands
- one hand on base
- other hand on arm
- do not let cord dangle to aviod tripping
what sould you clean lenses with
- lens paper and solution if needed before and after use
- clean oil immersion last
cleaning the microscope
- remove the slide
- clean lenses
- lower stage
- rotate to scanning objective because less damange
- before putting microscope away have teacher check it
- wrap cord
- replace dust cover
- put under proper location

Author

vanessasoto

233129

Card Set

bio 115

Description

lab notes

Updated

2013-09-06T00:31:34Z

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