-
microscopes depend on what 3 factors?
magnification, contrast, resolution
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a compound microscope has _______lenses which are the ________and _______lens.
2, ocular & objective
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With a typical compund microscope, the objective lens that will provide the largest, overall field of view is _____ for a total magnification of 40x.
4
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What is the total magnification of the ocular lens (10x) and the oil immersion lens (100x)?
1000x
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Most cell structures in on e microorganism are 3-5 nanometers apart. The detailed intracellular makeup of the microbe an be studied with the _____(sem or tem?)
TEM
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stains increase contrast so you can see the microorganism
better
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What are mordants?
not dyes, but intensify stain by increasing a cell's affinity for dye
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Stains are grouped into what 3 classes?
simple, differential, special
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What type of stain is a basic dye used to make cells visible?
simple
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What are the 3 steps used in differential stains?
primary stain, destaining, counterstaining
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Counterstaining is used to reveal the cell or parts of cells that have been _____
destained
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What 2 stains are used extensively in micro?
gram stain and acid fast stain
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Who developed the Gram stain?
Christian Gram
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What is the primary stain used in Gram stain?
crystal violet
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What is the mordant used in a Gram stain?
iodine
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What is the counterstain used in a Gram stain?
Saffranin
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After the 4 steps of the Gram stain are applied, a bacterium has a deep violet color, this is because it retained Genetial violet and safranin and is Gram______
positive
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A bacterium retains sfranin after decolorizing agent removes previously applied genetian violet of the Gram stain. The bacterium is Gram
negative
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Acid-fast stains are especially important test for the genus
mycobacterium
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the primary stain for the acid fast stain is
carbol fuchsin
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the counterstain for the acid fast stain is
methylene blue
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A petri dish is used to hold the nutrient
agar
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Coliform bacteria makes acid from ___and turns neutral red
lactose
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Blood agar is a _________growth medium.
?
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The purpose of serial dilution is to reduce the number of bacteria for counting purposes and for viewing colony
__________?
-
A pure culture consists of a _______ type of microorganism.
single
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The requirement for sterilizing most microorganisms by moist heat is _______for ________.
121*C for 15-20 mins
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Tuberculosis is caused by the genus
m. tuberculosis
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