-
What signals end of odontogenesis?
Eruption
-
What influences enamel biomineralization?
Stratum Intermedium, it expresses alkaline phosphatase
-
What signals inner DE to differentiate into ameloblasts?
predentin matrix deposition by odontoblasts in Late Bell Stage
-
What are knockout mice?
mice that have a gene removed from their embryonic stem cells in placed in fertilized egg, they lack the gene throughout their lifespan
-
What are transgenic mice?
Mice that have a gene inserted into the fertilized egg and is overexpressed where it is normally, it can be placed ectopically into organs where it is not normally expressed
-
Where do stem cells on dental pulp reside?
Perivascular niche
-
What is predentin rich in?
Type 1 Collagen
-
Mutation in what gene is responsible for the defects seen in DI Type 2 and 3?
DSPP Gene
-
What gene mutation causes DI Type 1?
Type 1 Collagen
-
What category of proteins is responsible in mineralization of dentin?
SIBLING
-
What are the three steps of ondontoblast differentiation?
Induction, Competence, and Terminal Differentiation
-
What is one of the hallmarks of terminal odontoblast differentiation?
Nuclear repolarization
-
What is the functional terminology for tooth development?
Initiation, Morphogenesis, Cytodifferentiation, Matrix Apposition
-
At what stage is the inductive potential transferred from the DE to the DP?
Bud stage
-
Dental papilla is termed ____ ____ at late bell stage.
dental pulp
-
What is the stellate recticulum?
Star shaped cell network that secretes high levels of GAGs which sequester fluid and GFs to expand the size of the enamel organ
-
What is the Trowell type system?
Mandibular and maxillary explants as well as molar organs can be cultured in enriched serum on a metal grid
-
What are bead implantation assays?
Heparin or agarose beads that are soaked in known concentrations of growth factor placed on separated mesenchyme, after 24 hours changes are observed
-
What are the two types of stem cells?
Embryonic and Postnatal
-
What is biologic redundancy?
When a gene is deleted in knockout mice the affect is not noticed since other genes within the same family cover for the gene deleted.
-
BMP 2,4 knockout mice undergo _____ _____ during _____ ______.
embryonic, lethality AND early, gestation
-
What happens to mice with absent Tgf-β1 gene?
Significant destruction of pulp and periradicular tissues as wells the hard tissues of the crown. No other TGF-β family members can substitute for the loss of β1
-
What characterizes cleidocranial dysplasia?
Open fontanels, defective clavicles, multiple supernumerary teeth that fail to erupt, tooth matrix defects
-
What gene mutation is suspected in cleidocranial dysplasia?
Runx2 a osteoblast transcription factor formerly known as Cbfa1 (core binding factor a1).
-
What is the morphogenic gradient in a developing tooth?
Most differentiated cells align at the tooth epithelial-mesenchyme interface and less-differentiated cells toward the core of the papilla
-
When do primary enamel knots appear?
Late bud stage
-
_____ is exclusively expressed in enamel knots
FGF-4
|
|
