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What is the deliberate growth of microorganisms in the lab?
culture
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a substrate or nutrient medium upon which microbes are cultured in a lab
can be liquid (broth), solid (agar), or semisolid depending upon the procedure & microbe
culture medium (media)
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a macroscopically visible mass of growth of microbes in or on a soild culture medium
represents a population of microbes from 1 living cell
central = older ; periphery = younger
colony
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unwanted organism, usually with reference to a pure culture of bacteria or some other microbe
contaminant
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microbial culture grown in culture tubes, petri dishes or other artificial environments
"in glass"
in vitro
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microbes grown on tissue or cell cultures such as chick or duck embryos, monkey kidney tissue, etc
used to cultivate obligate parasites suches as viruses or rickettsias
"in life"
in vivo
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an organism that must be a parasite in order to live
obligate parasite
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an organism tat lives on dead or decaying organic matter
saparophyte
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media that has an exact chemical composition that allows it to be reproduced easily
chemically defined (synthetic) medium
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a complex polysaccharide isolated from algae in the Indian Ocean
it is used in solid and semisolid media to serve as a substrate to grow microbes on
has a melting point of 100C and solidifies at 42C
it is indigestible by microorganisms and nutrients added to it can be extracted by them
agar
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medium to which reagents, dyes, etc. have been added to select for or against a certain group of bacteria
selective medium
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various vitamins, mineral and other substances hce veen added to promote the growth of fastidious microorganisms
those with extremely specific nutrient requirements
enriched medium
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medium with special formulations to determine if a microbe is utilizing a specific nutrient, produceing a specific product or is sensitive to an antibiotic
assay medium
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any medium that promotes the growth of a wide variety of microorganisms
enumeration medium
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medium that allows for distinction or differentiation between various bacteria
differential medium
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one which selects for and differentiates between a group of bacteria
selective & differential medium
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an organism that produces its own organic molecules (nutrients) from inorganic starting material
autotroph
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an organism that acquires its organic molecules (nutrients) from substances produced by other organisms
heterotroph
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bacteria that use light energy as an energy source and CO2 as a carbon source for metabolism
"photolithotrophic"
photoautotrophic
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bacteria that uses inorganic molecules as a carbon and energy source
chemoautotrophic
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bacteria that uses light as an energy source and organic molecules as a carbon source for metabolism
photoheterotrophic
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bacteria that uses organic molecules as a carbon and energy source
"chemoorganotrophic"
chemoheterotrophic
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