Lab 12 study guide

bacterial viruses

a complete set of a virus's hereditary material

to grow or produce by multiplication of parts

The lysis of bacteria growing on an agar plate produces clearing in the lawn of bacteria. These clearing are called plaques.

Since one infectious particle is responsible for one visible plaque, each particle is considered to be a plaque forming unit.

the cell the bacteria infects

• bacteriophage that looks like a spaceship
• DNA is packaged in the head and the tailhelps with the infection process

They carry the genetic information in all cells.

Viruses carry either RNA or DNA but never both. They are designated as either RNA or DNA viruses.

No. It is either an RNA or a DNA virus, but never both.

• adsorption
• penetration
• replication
• assembly
• release

tail fibers of the phage bind to the host's cell wall (attachment)

The enzymes present in the phage tail degrade the host cell wall and the viral nucleic acid passes through the hollow core into the host cel's cytoplasm.

the phage genome uses the cell's synthetic machinery for production of new phage components.

the phage components are assembled into mature virulent phage particles

enzymes lyse the host cell wall and allow the viruses to escape from the host

E. coli

DNA is packaged in the head

They are used to plate the dilution tubes

They are used for the dilution process

The E. coli is the host for the T4 bacteriophage

A sterile pipette must be used each time to accurately transfer the broth and not cross contaminate