Methyl Red tests for what?
- organisms capable of performing a mixed acid fermentation
- -overcomes phosphate buffer in medium to lower pH
- -Methyl red is red at pH 4.4., yellow at 6.2
- Positive = red (ferments acid)
- Negative = yellow
- orange = negative/inconclusive
Voges-Proskauer Test tests for what?
- test for ability to ferment glucose and quickly convert acid products to acetoin and 2,3-butanediol
- positive result: red
- negative: no color change
What is the "clean catch"?
Pee a little bit to flush out bacteria on skin, stop, then catch rest of urine in cup
What is a urinalysis?
Analysis of urine by physical, chemical, and microscopical means to test for the presence of disease, drugs, etc.
What information does a urinalysis dipstick provide?
- specific gravity
What is the purpose of the coagulase test?
differentiate staphylococcus aureus
- add organism to plasma on slide
- positive: agglutination in 2 minutes
What is your blood type based on?
What is present if you have type A blood?
Which antigens are present/what antibodies are in the plasma
A: A antigens, B antibodies in plasma
AB: A and B antigens, no antibodies (universal receiver)
O: No antigens, A and B antibodies (universal donor)
What is the universal donor? receiver?
what is the RH factor?
An antigen present on the blood cell (RH+)
- Someone who is RH+ can get RH+ or RH- blood
- Someone who is RH- can develop RH antibodies after receiving a transfusion of RH+ blood
How do you determine someone's blood type in lab?
Each well has either A antibodies, B antibodies or RH antibodies
If a well agglutinates, that antigen is present (i.e. if the well with A antibodies agglutinates, A antigens are present and they are sticking to the antibodies- which are not normally present in type A blood- therefor it is type A blood)
If it clumps, that's what type it is