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Large GPB with spores
(only spore forming bacteria that grows aerobically)
Spores oval in shape - do not swell beyond cell
May chain to resemble bamboo rods
sometimes V & L shaped
Growth on media for both types of bacillus spp.
SBA - 18-24 hours
: gray white frosted medusa head projections coming up
: beta hemolysis, gray white frosted
Catalase test for both bacillus spp
Motility for both species
Antibiotic therapy for both bacillus spp.
anthracis: Sensitive to penicillins
: flouroquinalones, tetracylcines and others.
: avoid beta lactams such as penicillins and cephalosporins they are less effective.
Clinical specimen for anthracis
blood, CSF, etc
What are the confirmatory identification for anthracis?
Capsule production on bicarbonate media
DFA (capsule antigen cell wall.
PCR - specific ID of DNA
India ink stain
B cereus is most noted for what type of infection?
Food poisoning - Is toxin mediated which is food that is contaminated with bacteria.
Less common: opportunisitic infection
Epidemiology/etiology for B. anthracis
Typically acquired through contact with anthrax-infected animals or animal products.
Cutaneous - direct contact - wool sorters disease.
Inhalation - aspiration of spore aerosol
: eating of contaminated meat.
NOT PERSON TO PERSON SPREAD
Epidemiology for B. cereus
Diarrheal - meat 24 hrs self limiting
Emetic - fried rice 10 hrs self limiting
Both caused by 2 distinct toxins
Opportunistic: ocular infection & wound infection