The flashcards below were created by user
nghiatrinh1991
on FreezingBlue Flashcards.
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What is aseptic technique?
To transfer living microbes from one place to another without contaminating yourself, others, the sterile medium and the surround
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What is the importance of aseptic technique?
To isolate one type of bacteria to get pure culture and avoid getting contaminated with pathogenic bacteria
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What is a culture?
A medium that contains living microbes
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What is a pure culture?
A medium that contains a single species
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What is a mixed culture?
A medium that contains one ore more species
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What does liquid/broth use for?
To grow fresh cultures, large volumes, in short amount of time
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What is the solid/agar medium use for?
- Agar slant, stock cultures, longer storage time.
- Plated media used for isolating species, quantifying bacterial densities
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What is morphologies? and give some example
- Morphologies are shapes.
- Ex: cocci (coccus-spheres)
- bacilli (bacillus-rods)
- spirilla (spirals)
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What is Pleomorphism?
- When one organism shows several shapes, irregular being normally rod-shaped.
- Ex: Corynebacterium xerosis
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What is the purpose of staining?
to provide contrast of the specimen against the background so we can view the detail of the cell under microscope.(Morphology, size, and arrangement).
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What is arrangement?
When bacteria present different types of division pattern.
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What are some type of arrangement?
Chain vs. cluster; diplococcus/ Diplobacilli; tetrad(4 cell together, flat) vs. sarcina( 4 -6 cells on each side, 3 dimension)
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What are stains?
Colored molecules which are chromogen dissolved in a solvent(water or ethanol)
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What is the portion of the chromogen that gives its color?
Chromophores
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What is the portion of the chromogen that is charged?
Auxochrome
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What are basic stain and what do they get attracted to?
- Basic stain has positive auxochrome that is attracted to the negatively charge cell surface.
- Ex of basic stain: methylene blue, crystal violet, safranin.
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What is a negative stain?
A negatively charge stain that repel the cell membrane, leaving the cell to appear uncolored on a color background
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What is the purpose of heat-fixing the cells?
Kills the bacteria and adhere the cells to the surface of the slide, avoid shrinkage of the cells.
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