What blood concentration method uses low-speed centrifugation to concentrate blood samples suspected of containing minimal numbers of parasites?
What blood concentration method is used for Leishmania and Trypanosoma detection?
What type of microscopic detection are quick and easy to perform and will allow trophozoite motility and helminth ova and larvae to be seen?
Saline wet mounts
What type of microscopic detection technique is useful for the detection of larvae, ova, and protozoan cysts in stool samples?
Iodine wet mounts
Name some of the permanent stained smears used for microscopic detection of stool, tissue, and blood parasites
Iron hematoxylin stain
Modified acid-fast stain
Modified trichrome stain - includes Weber green stain and Ryan blue stain
What permanent stain is used to detect Cryptosporidium and Isospora?
Modified acid-fast stain
What permanent stain is used for microsporidia and what color will they be?
Modified trichrome stains- includes Weber green stain and Ryan blue stain
Weber green stain - microsporidia will stain pink
Ryan blue stain - microsporidia will also stain pink but the background is blue
What collection method is used to collect Enterobius vermicularis (pinworm eggs) from the perirectal area?
Cellophane (Scotch) tape
What is the term for a tapeworm?
What is the term for a symbiotic relationship beneficial to one member and harmless to another?
What is the term for a thick-walled stage of protozoa resistant to adverse conditions?
What is the term for a host supporting the adult or sexual phase of a parasitic life cycle?
What is the term for a parasite found on the surface of a host?
What is the term for a parasite found inside a host
What is the term for blood or tissue roundworms?
What is the term for containing ova?
What is the term for worms that include nematodes (roundworms), cestodes (tapeworms), and trematodes (flukes)?
What is the term for an organism capable of self-fertilization?
What is the term for the larval stage of Echinococcus granulosus?
What is the term for a host containing the asexual phase of a parasite?
What is the term for the junenile stage of a parasite?
What is the term for the symbiotic relationship beneficial to both species?
What is the term for roundworm?
What is the term for an encysted form of an egg?
What is the term for a symbiotic relationship in which one member benefits at the expense of another member (the host)?
What is the term for an association between 2 or more organisms of a different species?
What is the term for fluke or flat worm?
What is the term for the developmental stage of protozoa?
What is the term for an animal infection or disease that humans accidently acquire?
What stage of Protozoa is the infective stage?
What is the term for the development of a cyst into a trophozoite?
What is the term for the development of a trophozoite into a cyst?
What is the term for the rod-shaped, RNA containing structure found in the cytoplasm?
Contains 1-4 nuclei
- CYTOPLASMIC INCLUSION: INTACT RBC'S THAT HAVE BEEN PHAGOCYTIZED
- KARYOSOME: SMALL AND CENTRAL
Cyst: NUCLEI: 2-4
- KARYSOME: LARGE AND ECCENTRIC (OFF TO THE SIDE)
- PERIPHERAL CHROMATIN: COARSE AND IRREGULAR, GRANULAR (MAY NOT BE ALL THE WAY AROUND)
- SIZE: 10-50µ
- NUCLEI: 4-8 (ONLY PROTOZOA WITH MORE THAN 8)
- CYTOPLASM: LARGE, CENTRAL, FLUID-FILLED VACUOLE SURROUNDED BY A LAYER OF CYTOPLASM CONTAINING THE NUCLEI
- NUCLEI: ONE TO SEVERAL
Causes giardiasis (a form of traveler's diarrhea)
- contain 4 nuclei
- PROMINENT AXOSTYLE (FIBER THICKENING THROUGH THE MIDDLE OF THE CYST)
- OVAL SHAPE
- Pear shaped,
- contain 2 oval shaped nuclei,
- has 4 flagella
- 2 VENTRAL SUCKING DISCS
- MOTILITY: FALLING LEAF MOTION
- karyosome is large
- centrally located and cytostome is well defined
- LEMON SHAPED
- ANTERIOR HYALINE PROTRUSION
- SINGLE NUCLEUS
- SHEPERD'S CROOK
Has cytoplasm that is vacuolated with bacterial inclusions
MOTILITY: BROAD PSEUDOPODIA
- 2 NUCLEI
- KARYOSOME IS CLUSTER OF 4-8 GRANULES
-MOTILITY: CILIATED SURFACE (ONLY CLINICALLY SIGNIFICANT CIILIATE)
-NUCLEUS: MACRONUCLEUS AND MICRONUCLEUS
-CYTOSOME: A FUNNEL-SHAPED PRIMITIVE MOUTH
-CYST WALL: SMOOTH
-NUCLEUS: ONE MACRO & MICRONUCLEUS
LARGEST INTESTINAL PROTOZOA
Diagnosis made with microscopic detection of acid-fast oocysts in stool or small bowel mucosal epithelial cells
Diagnosis is made by exam of stained fecal smears - oocysts will stain with modified Kinyoun's acid-fast stain and are 8-10 micro-meters
What protozoa is diagnosed by microscopic exam of stool for oocysts by wet mounts and/or acid-fast or auromine-rhodamine stains?