Hematology

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What is the formula for calculating a manual WBC count using a hemacytometer?
What is the formula for calculating a correction for the presence of NRBCs?
What is the formula for calculating a manual platelet count, using a hemacytometer?
What type of blood cell enumeration:
When cells pass through an aperature with an electrical current flowing through simultaneously
Cells do not conduct current but rather they change electrical resistance, which is then counted as voltage pulses

Electrical impedance
What type of blood cell enumeration:
Uses a flow cytometer with laser to measure light scattering properties of cells
Forward angle light scatter measures cell size
Side angle light scatter provides info on cell granularity and lobularity

Light scattering optical method
What type of automated cell count error occurs when the WBC count exceeds instrument linearity limits?
- Increased:
- Hgb
- MCH
- MCHC
What type automated cell count error occurs when the glucose is over 600 mg/dL?
- Increased MCV
- Increased HCT
- Decreased MCHC
What type automated cell count error occurs when there is a cold agglutinin?
- Increased MCV
- Increased MCH
- Increased MCHC
- Decreased RBC
- Decreased HCT
What type of automated cell count error occurs when there is lipemia?
- Increased:
- Hgb
- MCH
- MCHC
What is the Rule of three calculation to help identify automated cell count errors?
- RBC x 3 = Hgb
- RBC x 9 = HCT
- Hbg x 3 = HCT
What is the usual cell size and which peak of the WBC histogram is for lymphocytes?
- 35-90 fL
- 1st peak
What is the usual cell size and which peak of the WBC histogram is for monocytes, reactive lymphs, and immature WBCs?
- 90-160 fL
- 2nd peak
What is the usual cell size and which peak of the WBC histogram is for granulocytes?
- 160-450 fL
- 3rd peak
What is the calculation for blood oxygen capacity?
What is the reference method to measure all hemoglobins except for sulfhemoglobin?

Cyanmethemoglobin
What type and name of the stain is used to demonstrate reticulum in reticulocytes?

Surpravital stain - New methylene blue
What is the calculation for the relative count of retics?
What is the calculation for the absolute count of retics?
What is the reference range for ESRs?
- 0-20 mm/hr
- Age and sex dependent
What causes an ESR to be increased?
- Chronic inflammatory conditions
- Rheumatoid arthritis
- Pregnancy (increased fibrinogen)
- Bacterial infections
- Malignancy
- Tissue damage
- Multiple myeloma
- Waldenstrom macroglobulinemia
- Severe anemia
What causes and ESR to be decreased?
- Polycythemia
- Sickle cell anemia
- Spherocytosis
- Other conditions with poikilocytosis (prevents rouleaux formation)
What is the test used to detect the presence of fetal cells in maternal circulation during problem pregnancies?

Kleihauer-Betke method (Hbg F)
What is the reference range of Hgb F in newborns?

70-90% Hgb F
What test is used for Sickle cell (Hgb S) and what is the result?
- Solubility test
- Hgb S is insoluble when combined with a reducing agent (sodium dithionite)
What is the procedure used for the identification of normal and abnormal hemoglobins?

Hemoglobin electrophoresis
Name the 2 types of electrophoresis
- Cellulose acetate at pH 8.6
- Citrate agar at pH 6.2
What type of electrophoresis is used to differentiate Hgb C?

Citrate agar at pH 6.2
What type of electrophoresis is used to differentiate Hgb S?

Citrate agar at pH 6.2
Name the major components of a flow cytometer
- Fluidics
- Optics
- Electronics

Author

ncrook

212965

Card Set

Hematology

Description

Tests

Updated

2013-05-01T23:48:13Z

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