Oxidase Test

What is the oxidase test purpose?

To identify bacteria that contain cytochrome c oxidase
What is the function of cytochrom C oxidase?

Carry the electron from cytochrom C to O2 in Electron transport chain. reducing O2 to H2O

Can also catalyze the reduction of cytochrome C by a chromogenic reducing agent
Is Enterobacteriaceae oxidase (+) or (-)?

Oxidase (-)
What do we call chemical that develop color as they become oxidized?

Chromogenic reducing agent
What is the chromogenic reducing agent used in oxidase test?

Tetramethyl-p-phenylenediamine(TMPD)
What does it mean if the bacteria turn purple when TMPD is added to the bacteria?
- The bacteria contain cytochrom c oxidase and that oxidized cytochrome c.
- The oxidase test is positive
What happen when the bacteria is colorless when TMPD is added?

Phenylenediamine is reduced

Author

nghiatrinh1991

212630

Card Set

Oxidase Test

Description

Microbio lab oxidase test

Updated

2013-04-10T12:37:28Z

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