Oxidation-Fermentation

Differentiate bacteria based on their ability to oxidative and/or fermentative metabolism of carbohydrates

CO2,H2O, and energy

Fermentation doesn't use O2 as final electron acceptor. Pyruvate and other organic intermediates reduced to organic acids, gas, or alcohol

To prevent alkaline product from peptone to neutralize weaker acids made from oxxidation of the carbohydrate

Bromthymol blue

It turn yellow at pH 6, green at pH 7.1 and blue at pH 7.6

Allow us to see motility

Mineral oil promote an anaerobic growth and fermentation. (Inhibit oxidation). Air can't diffuse through the oil to the medium

If the bacteria turn the O-F tube yellow in aerobic and anaerobic condition

If the bacteria only turn the O-F tube yellow in either aerobic or anaerobic condition

Bacteria can't oxidize or ferment the sugar

Alkaline product from amino acid degradation

Fermentative Enterobacteriaceae from the oxidative Pseudomonas and Bordetella, and the non reactive Alcaligenes and Moraxella

breakdown of molecules (releases energy)

formation of molecules (require energy, store energy)

Bacteria that can degrade amino acid(turn the O-F tube basis and blue)