What is the differential ingredient in Bile Esculin Hydrolysis Test?
Esculin
What is the substrate of Bile Esculin Agar?
glucose and esculetin
Is the substrate easily hydrolyzed under acidic condition?
Yes
What does bacteria need to hydrolyze esculin?
Bile
What is the selective agent in Bile Esculin?
Bile
What is the indicator of iron?
Ferric citrate
Which bacteria group is bile esculin positive? Bile esculin negative?
Enterococci
Streptococcus pneumoniae and Streptococcus pyogenes
Describe the process of a positive bile esculin test
Bacteria can hydrolyze esculin and split it into esculetin. Esculetin combine with ferric citrate and form dark brown phenolic iron complex. Show up dark.
Where can you find Enterococci, Enterococcus faecalis?
Gastrointestinal tract, vagina, oral cavity
Does Enterococci and Enterococcus faecalis need O2?
NO, just area with nutrients
Are Enterococci and Enterococcus faecalis highly resistant to antibiotic?
Yes
How do Enterococci and Enterococcus faecalis enter the blood stream?