Blood Agar

Is Blood Agar a differential media or selective media?

Differential based on the ability to hemolyze red blood cells(RBCs)
What is Blood Agar made of?

Sheep blood and tryptic soy agar base
What does the Blood Agar used for?

Detect the hemolyticability of Gram-Positive cocci(Streptococcus)
What is hemolysin?

Check lab manual
What is exotoxins?

Check lab manual
Which type of hemolysis has a complete lysis of RBCs and hemoglobin?

Beta-hemolysis
Which type of hemolysis has partial destruction of RBCs?

Alpha hemolysis
Which type of hemolysis does not destroy RBCs?

Gamma hemolysis
How do you detect Beta, Alpha, and Gamma hemolysis on Blood agar?
- Beta hemolysis=clearing of the medium around colony
- Alpha hemolysis=Greenish discoloration of the agar around the colonies
- Gamma hemolysis=Simple growth with no change to medium
What is Streptolysins?

Hemolysin produced by Streptococcus sp.
What is streptolysin O? What is Streptolysin S?

Hemolysins produced by Streptococcus sp. under anaerobic condition

Hemolysin produced by Streptococcus sp. under aerobic condition
Which bacteria do Blood Agar differentiate between?

Differentiate between Streptococcus and Enterococcus

Author

nghiatrinh1991

212482

Card Set

Blood Agar

Description

Blodd Agar

Updated

2013-04-09T22:30:57Z

Show Answers