PBMCs with Adenovirus show resistance to HIV-1 infection.txt

Identify characteristics of PMBC-derived dendritic cells and their RESISTANCE to HIV-1 INFECTION by using adenovirus vector to insert various genes

• Addn of:
• 1. a sequence modified CCR5delta32 gene: interferes with CCR5 and CXCR4 fxn
• 2. transduction of siRNA to block CCR5 expression
• anti-HIV siRNA genes
• 3. HIV-1 pol
• 4. HIV-1 int
• 1 and 2 role: BLOCK VIRAL ENTRY
• 3 and 4 role: SUPRESS HIV replication

HIV-1 infection resistance was observed when using cells with these recombinant adenoviral vectors

• CCR5delta32 expression INCREASED
• CCR5 and CXR4 expression DECREASED: Possibly due to inhibition by CCR5siRNA or delta32

• Used p24 ELISAs to monitor replication
• Results: in UNTRANSFECTED cells, p24 INCREASED vs. Ad-R5delta32siHIV showed NO change of p24.
• Interpretation: INHIBITION of viral replication seen with Adenoviral infected PBMCs

• Magi cells have b-galac gene under control of HIV LTRs
• Magi cells are susceptible to HIV strain IIIB infection
• After transfection with adenovirus, Magi cells did NOT show susceptibility to HIV (Blue from b-galac decreased)

• When looking at the DCs (Mature dendritic cells) the adenoviral infected cells showed INCREASE in these cells vs control
• Interpretation: The adenovirally infected cells can INDUCE T-cell IMMUNITY

surface marker used as a measure to see if DCs with adenovirus were different than the control DCells