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Catalase positive
Staphylococcus
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Catalase Negative
Sterptococcus
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What does coagulase convert?
Fibrinogen to fibrin
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What organism is Coagulase positive?
S. aureus
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What do you verify the Staph tests with?
Mannitol and DNAse
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What are the Mannitol and DNAse results for S. aureus?
Both positive
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What is S. aureus susceptibility to Novobiocin?
greater than or equal to 17mm
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If the Coagulase test is negative what is the next test?
Novobiocin
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What Staph is novobiocin resistant?
S. saprophyticus
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What Staph is Novobiocin susceptible?
S. epidermidis
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What is the Mannitol and DNAse results for S. epidermidis?
Both negative
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What are the Mannitol and DNAse test results for S. saprophyticus?
Mannitol+/- DNAse -
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What organisms have beta hemolysis?
S. pyogenes, S. agalactiae
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What organisms are alpha hemolysis?
S. pneumoniae, S. oralis
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What organisms are non hemolytic?
S. bovis, and E. faecalis
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What drug is used to during beta hemolysis?
Bacitracin (A disc)
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What drug is used during Alpha hemolysis?
Optochin (P disc)
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What drug is used for non hemolytic?
Bile Esculin
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What organism is susceptible to Bacitracin?
S. pyogenes
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If the strep is Bacitracin resistant what is the next test?
cAMP test
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What produces a positive cAMP test?
S, agalactiae
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What is optochin susceptible?
S. pneumoniae
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What is optochin resistant?
S. oralis?
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What is the next test if the Bile exculin test is positive?
NaCl or SF
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What is NaCl or SF positive?
E. faecalis. Turbidity or Yellow
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What is negative for NaCl or SF?
S. bovis
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What does direct ELISA test for?
Antigen detection
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What does indirect ELISA test for?
antibody detection
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What does immunodiffusion or ouchterlony assay allow?
Detects identity, partial identity or non identity of antigens/antibodies
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What does immunofluorescence assay allow?
use a direct assay with only ONE reagent or indirect with TWO reagents
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What is Immunoflouescence assay (FA) used for primarily?
Antigen detection
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What is the conformation of strep?
Gram positive cocci in chains
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What kind of media should you plate strep on?
Blood and chocolate
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What is Lancefield?
groups divided by serological testing of extractable carbohydrates
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What are the Lancefield groups?
A,B,C,D
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What groupd causes reumatic fever
Group A
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What disease does Group B cause?
UTI in woman and diseases of newborns
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What diseases does Group C cause?
Pathogens in animals. occasionally sinusitis in man
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What disease does Group D cause?
Found in intestinal tract. Endocarditis UTI's and infections in wound
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What are the virulence factors of Strep?
Hemolysins, Leucocidins, Erythrogenic toxin(A), Hyaluronidase, streptokinase, nucleases
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What is the conformation of Staph?
Gram positive cocci in clusters
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What is the indicator in DNAse?
Methyl green
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What does S. aureus cause?
acne, boils, pneumonia, bone inflammation, endocarditis, food poisoning
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What are the virulence factors of Staph?
Leukocidins, Hemolysins, Coagulase, Enterotoxin, DNAse and Lipases
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How many times more likely are UTI's in females?
14
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What are the normal mouth flora?
S. aureus, S. epidermidis, strep. mutans
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What is the Snyder Test used for?
Determining the hosts susceptibility to dental carries
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What is the indicator in the Snyder test?
Cresol Green
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What is immunology?
ability of an individual to resist infection by a particular microorganism due to natural or acquired defense mechanisms
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What is a flourescence activated cell sorter
type of flow cytometry in which different cells within a suspension are detected based on flourescent antibody tag
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Antiseptics
used on not in living tissue
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Disinfectants
used on inanimate objects to inhibit growth
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Chemotherapeutic agents
Chemicals that destroy bacteria in living tissue
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