What are the 2 ways genetic info in prokaryotes can be transferred?
Vertically or horizontally
Vertical gene transfer is transfer of genetic info fr where to where?
Parent cell to daughter cell
Horizontal gene transfer is the transfer of DNA fr where to where?
Donor cell to recipient cell
Describe transformation.
Uptake of free DNA fragment fr surrounding environment & the expression of genetic info in the recipient cell
DNA transformation involves the transfer of DNA via
C) Naked DNA in solution
The transfer of genetic material between bacteria in direct physical contact is called
A) conjugation
The transfer of a naked fragment of DNA between bacteria is called
A) transformation
The transport of bacterial DNA to other bacteria via bacteriaphages is called
A) transduction
Horizontal transfer can best be described as:
C) the transmission of genetic information fr one independent, mature organism to another
Plasmids can best be described as:
A) small circular DNA molecules that can exist independently of chromosomes commonly found in bacteria
F factor plasmids play a major role in what bacterial process?
C) conjugation
This type of plasmid makes the host more pathogenic
C) virulence plasmid
Mobile genetic elements that carry the genes required for integration into host chromosomes
B) transporons
Which of the following are true in regards to F+ x F- mating events?
C) DNA is transferred fr F+ to F- cells
A bacterial cell that is able to take up naked DNA is said to be
B) competent
The relationship between a virus and host where no new viral particles are produces and the viral genome is replicated along with host chromosome
C) lysogeny
Which of the following can be used as a tool by microbial genetisists?
D) all of the above
Recombination of virus genomes occurs
A) when 2 viruses w/homologous chromosomes infect a host cell simultaneously
These are bacterial proteins that destroy other bacteria
C) bacteriocins
Transformation was first described by whom, when?
Frederick Griffith in 1928
___ is the ability of a recipient cell to take up DNA fr the environment.
Competence
What is the term given to cell to cell contact for horizontal gene transfer?
Conjugation
In conjugation, how does the donor cell transfer DNA to the recipient?
Donor cell forms a conjugation pilus to make contact with the recipient
What process involves viruses as agents for the horizontal transfer of DNA?
Transduction
How are chromosomal DNA fragments carried in transduction?
A virus or bacteriophage carries the fragment fr donor to recepient
What 3 things do virulent phages do in the lytic cycle?
Destroy the host chromosome
Replicate themselves
Destroy the cell
In the ___ cycle, temperate phages integrate their DNA into host chromosomes as a ____.
Lysogenic
Prophage
A recombinant DNA molecule contains DNA fragments....
Spliced together fr 2 or more organisms
What does genetic engineering involve?
Changing genetic material in an organism to alter its traits or products
How do genetic engineers obtain specific fragments of DNA?
By cutting short stretches of nucleotides w/a restriction endonuclease
They are then joined by DNA ligase
What is a DNA probe?
Single strand of DNA that recognize & bind w/a specific nucleotide sequence of a pathogen
What is microbial genomics?
The discipline of sequencing, analyzing and comparing microbial genomes
The ability to ID unculturable organisms is opening up the new discipline of ___.
Metagenomics
Which of the following involves DNA polymerase activity?
A) Replication
Which of the following processes requires a DNA ligase to seal Ocazaki fragments?
C) Replication
Which of the following processes is DNA dependent RNA synthesis process?
C) Transcription
RNA polymerase starts starts ___ at a ctrl sequence called a promoter found on the template strand?
A) Transcription
The RNA polymerase transcribes the template, substituting ___ for ___ where adenine appears in the DNA template strand.
Uracil for thymine
What is DNA recombination?
A natural mechanism for DNA transfer fr one microorganism to another
Which of the following includes the attachment of viral DNA to the bacterial chromosome?
B) transduction
Which of the following involves a transfer of genes via direct contact?
A) Conjugation
Which of the following occurs in generalized and specialized forms?
C) Transduction
Which of the following requires competent cells be available?
A) Transformation
Which of the following carries viral genes fr bacterial chromosomes?
A) Transduction
Which of the following was first observed by Griffith in pneumococci?
A) Transformation
Which of the following involves F+ cells as donor cells?
A) Conjugation
Which of the following utilizes a pilus?
B) conjugation
Which occurs when naked pieces of DNA are taken up fr the environment?
C) Transformation
Which involves an unencapsulated bacterium becoming encapsulated?
A) Transformation
Which follows the lytic cycle of a bacteriophage?
A) transduction
Which involves plasmids moving between live bacteria?
B) Conjugation
A viral genome integrated into a host genome is known as a ___.
Prophage
F⁺ cell
Plasmid
Prophage
A cell capable of being transformed is considered to be ___.
Competent
Which is a cell w/conjugation pilus; plasmid in the cytoplasm?
Plasmid
F⁺ cell
Prophage
F⁺ cell
The ___ ___ are events in a cell as virus particles are replicated and released.
Lytic Cycle
A hollow structure for DNA transfer is known as a ___.
Pilus
Which is a cell that conjugates; donates bacterial DNA genes?
Prophage
Hfr
F⁺ cell
Hfr
A condition in which a viral infection does not cause immediate cell death is known as ___.
Lysogeny
A closed circle DNA w/few genes and are nonessential to the cell is known as a what?
Plasmid
T or F; Griffith's landmark experiments of 1928 demonstrated that
bacterial recombinations with viruses take place so long as the viruses are
lysogenic.
False
Response: Griffith's landmark experiments of 1928 demonstrated that an unknown transforming substance passed from dead S strain to live R strain bacteria.
T or F; Genetic recombination is the alteration of the bacterial
chromosome by a change in its nucleotide sequence.
False
Response: Genetic recombination is the transfer of plasmid or chromosomal DNA fragments laterally from donor to recipient cell.
T or F; Transformations are generally more common than conjugations because DNA passes easily through the recipient cell's wall and membrane.
False
Response: Transformations are generally less common than conjugations because DNA does not pass easily through the recipient cell's wall and membrane.
T or F; Transformation appears to be the major mechanism for antibiotic transfer.
False
Response: Conjugation appears to be the major mechanism for antibiotic transfer.